FBXW11 Monoclonal antibody, PBS Only

FBXW11 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F5A10

Cat no : 68090-1-PBS

Synonyms

BTRC2, BTRCP2, Fbw11, FBW1B, FBXW11, FBXW1B, Homologous to Slimb protein, Hos, KIAA0696

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Validation Data Gallery

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  WB analysis using 68090-1-Ig (same clone as 68090-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68090-1-Ig (FBXW11 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68090-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 68090-1-Ig (same clone as 68090-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68090-1-Ig (FBXW11 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68090-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 68090-1-Ig (same clone as 68090-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 68090-1-Ig (FBXW11 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68090-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 68090-1-Ig (same clone as 68090-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 68090-1-Ig (FBXW11 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68090-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 68090-1-Ig (same clone as 68090-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68090-1-Ig (FBXW11 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68090-1-PBS in a different storage buffer formulation.

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Product Information

68090-1-PBS targets FBXW11 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: FBXW11 fusion protein Ag3761
• Full Name: F-box and WD repeat domain containing 11
• Calculated Molecular Weight: 542 aa, 61 kDa
• Observed Molecular Weight: 58-62 kDa
• GenBank Accession Number: BC026213
• Gene Symbol: FBXW11
• Gene ID (NCBI): 23291
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

FBXW11 (also known as HOS or β-TrCP2) is a member of F-box family proteins and plays critical role in regulating the ubiquitination of phosphorylated substrates. Abnormal expression of several FBXW11 is involved in the modulation of various biological events, such as cell cycle, differentiation, migration, inflammation, and apoptosis, through targeting multiple different substrates. For instance, FBXW11 could bind to the phosphorylated IκB and β-catenin, promoting their degradation via the ubiquitin-proteasome system. In addition, FBXW11 expression is markedly increased in mouse skin tumors and promotes tumor growth by activating the NF-κB signaling (PMID: 33640602). FBXW11 has 3 isoforms with the molecular mass of 58-62 kDa.