FBXO6 Polyclonal antibody

FBXO6 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 11830-1-AP

Synonyms

F box only protein 6, F box protein 6, F box/G domain protein 2, FBG2, FBS2, FBX6, Fbx6b, FBXO6

---

Validation Data Gallery

• 

  WB analysis using 11830-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 11830-1-AP (FBXO6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 11830-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11830-1-AP (FBXO6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 11830-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11830-1-AP (FBXO6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: mouse lung tissue
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11830-1-AP targets FBXO6 in IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FBXO6 fusion protein Ag2400
• Full Name: F-box protein 6
• Calculated Molecular Weight: 293 aa, 34 kDa
• Observed Molecular Weight: 35-40 kDa
• GenBank Accession Number: BC020880
• Gene Symbol: FBXO6
• Gene ID (NCBI): 26270
• RRID: AB_2104709
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FBXO6(F-box only protein 6 ) is also named as FBG2, FBS2, FBX6 and belongs to the F-box protein family. It can regulate Chk1 ubiquitination and degradation in both normally cycling cells and during replication stress. This protein is mainly detected in the cytoplasm in both cultured cells and in tumor tissues. Its expression is a predictive biomarker of tumor responsiveness to the important anticancer drugs.(PMID:19716789). FBXO6 can inhibit the anaphase promoting complex (cyclosome APC) and prevent mitotic exit in cytostatic factor (CSF) arrested eggs as a mitotic regulator.

Publications

Species	Application	Title
mouse		Cell Signal; Musashi-2 binds with Fbxo6 to induce Rnaset2 ubiquitination and chemokine signaling pathway during vascular smooth muscle cell phenotypic switch in atherosclerosis; Authors - Tao Zhang