FBXO5 Polyclonal antibody, PBS Only

FBXO5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10872-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of HEK-293 using 10872-1-AP (same clone as 10872-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10872-1-AP (FBXO5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10872-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human ovary using 10872-1-AP (same clone as 10872-1-PBS)

  human ovary tissue were subjected to SDS PAGE followed by western blot with 10872-1-AP (FBXO5 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10872-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 10872-1-AP (same clone as 10872-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 10872-1-AP (FBXO5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10872-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 10872-1-AP (same clone as 10872-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 10872-1-AP (FBXO5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10872-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 10872-1-AP (same clone as 10872-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10872-1-AP (FBXO5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10872-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse kidney using 10872-1-AP (same clone as 10872-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10872-1-AP (FBXO5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10872-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat liver using 10872-1-AP (same clone as 10872-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 10872-1-AP (FBXO5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10872-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 10872-1-AP (same clone as 10872-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10872-1-AP (FBXO5 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 10872-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 10872-1-AP (same clone as 10872-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10872-1-AP (FBXO5 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 10872-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 10872-1-AP (same clone as 10872-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10872-1-AP (FBXO5 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 10872-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 10872-1-AP (same clone as 10872-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10872-1-AP (FBXO5 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 10872-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 10872-1-AP (same clone as 10872-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10872-1-AP (FBXO5 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 10872-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 10872-1-AP (same clone as 10872-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10872-1-AP (FBXO5 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 10872-1-PBS in a different storage buffer formulation.

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Product Information

10872-1-PBS targets FBXO5 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FBXO5 fusion protein Ag1296
• Full Name: F-box protein 5
• Calculated Molecular Weight: 54 kDa
• Observed Molecular Weight: 56 kDa, 45 kDa
• GenBank Accession Number: BC018905
• Gene Symbol: FBXO5
• Gene ID (NCBI): 26271
• RRID: AB_2247034
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

FBXO5, also named as EMI1 and FBX5, is a key regulator of the normal cell cycle. It is a a zinc finger protein expressed by a variety of adult tissues and especially in proliferating Ki-67-positive cells. It binds to the APC activators CDC20 and FZR1/CDH1 to prevent APC activation. FBXO5 can also bind directly to the APC to inhibit substrate-binding.