FBXO38 Recombinant antibody

• 

  WB analysis using 83509-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83509-5-RR (FBXO38 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 83509-5-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FBXO38 antibody (83509-5-RR, Clone: 240513A4 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

• 

  Affinity and Kinetic Characterization of 83509-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83509-5-RR against Human FBXO38 were performed. The affinity constant is 0.233 nM.

FBXO38 {ptg:Brand} Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 83509-5-RR

Clone No.240513A4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

SP329, MOKA, Fbx38, F box protein 38, F box only protein 38

---

Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, K-562 cells, Jurkat cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83509-5-RR targets FBXO38 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: FBXO38 fusion protein Ag35444
• Full Name: F-box protein 38
• Calculated Molecular Weight: 134kDa，1188aa
• Observed Molecular Weight: 125kDa, 140 kDa, 150kDa
• GenBank Accession Number: XM_005268513.1
• Gene Symbol: FBXO38
• Gene ID (NCBI): 81545
• RRID: AB_3671133
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q6PIJ6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

F-box-containing protein 38 (FBXO38), a member of the F-box family, was initially discovered as a co-activator of the transcription factor Krüppel-like factor 7 (KLF7) (PMID: 14729953). FBXO38 acts as a substrate-binding receptor for multi-subunit ubiquitin ligase SKP1-CUL1-FBXO38 (PMID: 35769260) . FBXO38 is an E3 ligase of PD-1 that mediates Lys48-linked poly-ubiquitination and subsequent proteasome degradation (PMID: 30487606).