FBXO38 Polyclonal antibody

• 

  WB analysis using 31282-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31282-1-AP (FBXO38 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

FBXO38 Polyclonal Antibody for WB, ELISA

Cat No. 31282-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

SP329, MOKA, Fbx38, F box protein 38, F box only protein 38

---

Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells, NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31282-1-AP targets FBXO38 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FBXO38 fusion protein Ag35444
• Full Name: F-box protein 38
• Calculated Molecular Weight: 134kDa，1188aa
• Observed Molecular Weight: 140 kDa, 107 kDa
• GenBank Accession Number: XM_005268513.1
• Gene Symbol: FBXO38
• Gene ID (NCBI): 81545
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q6PIJ6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

F-box containing protein 38 (FBXO38; also known as MoKA) is a substrate receptor for SKP1-CUL1-dependent ubiquitin ligase (SCF). Initially, it was discovered as a modulator of transcription factor Krüppel-like factor 7 (KLF7) activity (PMID: 14729953). In addition, FBXO38 stability is controlled via ubiquitin-specific peptidase 7 (USP7), and FBXO38 protein expression was shown to be required for proper cytokinesis (PMID: 30804394). However, as a ubiquitin ligase, SCFFBXO38 is still insufficiently characterized. So far, the only potential substrate identified is programmed cell death 1 (PD-1) (PMID: 30487606, PMID: 35813202).