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FBXO31 Polyclonal antibody, PBS Only

FBXO31 Polyclonal Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

Cat no : 27294-1-PBS

Synonyms

FBXO14, FBX31, FBX14, F-box only protein 31, F box protein 31

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using FBXO31 Polyclonal antibody (27294-1-AP)

    WB analysis of mouse brain using 27294-1-AP (same clone as 27294-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 27294-1-AP (FBXO31 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27294-1-PBS in a different storage buffer formulation.

Product Information

27294-1-PBS targets FBXO31 in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FBXO31 fusion protein Ag24867
Full Name F-box protein 31
Observed Molecular Weight 49 kDa, 60-70 kDa
GenBank Accession NumberBC012748
Gene Symbol FBXO31
Gene ID (NCBI) 79791
RRIDAB_2880833
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

FBXO31, also known as F-box protein 31, is a substrate recognition protein of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex. It plays a crucial role in mediating the ubiquitination and subsequent degradation of target proteins through the ubiquitin-proteasome system. In pancreatic cancer, FBXO31 is upregulated and promotes cancer progression by targeting proteins like SIRT2 for degradation (PMID: 38216561). In endometrial cancer, FBXO31 acts as a tumor suppressor by regulating the ubiquitination of OGT (O-GlcNAc transferase), which affects cellular O-GlcNAcylation levels (PMID: 39894887). FBXO31 Is a reader of C-terminal amides, which ubiquitylates CTAPs for subsequent proteasomal degradation. A dominant de novo D334N mutation in FBXO31 alters FBXO31 substrate recognition and CTAP clearance, causing some important proteins to be degraded and clinical intellectual impairment or diplegic spastic cerebral (PMID: 39880951).