FBXO27 Polyclonal antibody

• Featured Product
• KD/KO Validated

FBXO27 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, ELISA, IF

Conjugate

Unconjugated

Publications

2

Cat no : 14570-1-AP

Synonyms

FBX27, F-box/G-domain protein 5, F-box only protein 27, FBG5

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Validation Data Gallery

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  WB analysis of Panc-1 cells using 14570-1-AP

  FBXO27 expression in cells. Each whole cell lysate containing 15 ug protein was analyzed by immunoblotting using FBXO27 (14570-1-AP: 1:2000). Knock-down experiment confirmed that the pointed band showed FBXO27. (Data from Dr. Yukiko Yoshida, Ubiquitin Project, Tokyo Metropolitan Institute of Medical Science.).

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  IP experiment of PANC-1 using 14570-1-AP

  IP result of anti-FBXO27 (IP:14570-1-AP, 4ug; Detection:14570-1-AP 1:600) with PANC-1 cells lysate 1330 ug.

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  IHC staining of human kidney using 14570-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14570-1-AP (FBXO27 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 14570-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14570-1-AP (FBXO27 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: PANC-1 cells
• Positive IP detected in: PANC-1 cells
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 2 publications below

Product Information

14570-1-AP targets FBXO27 in WB, IHC, IP, ELISA, IF applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FBXO27 fusion protein Ag6157
• Full Name: F-box protein 27
• Calculated Molecular Weight: 32 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC030060
• Gene Symbol: FBXO27
• Gene ID (NCBI): 126433
• RRID: AB_2104422
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FBXO27 is a glycoprotein-specific F-box protein that is part of the SCF (SKP1/CUL1/F-box protein) ubiquitin ligase complex, and SCFFBXO27 ubiquitinates glycoproteins in damaged lysosomes will regulate autophagic machinery recruitment (PMID: 28743755).Unlike F-box proteins in other SCF complexes, FBXO27 is subject to N-myristoylation, which localizes it to membranes, allowing it to accumulate rapidly around damaged lysosomes.

Publications

Species	Application	Title
human	WB,IF	Proc Natl Acad Sci U S A; Ubiquitination of exposed glycoproteins by SCF(FBXO27) directs damaged lysosomes for autophagy.; Authors - Yukiko Yoshida ; KO Validated
human	WB,IF	Autophagy; TRIM16-mediated lysophagy suppresses high-glucose-accumulated neuronal Aβ; Authors - Chang Woo Chae