FBXO22 Polyclonal antibody

• Featured Product
• KD/KO Validated

FBXO22 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

27

Reviews (1)

Cat no : 13606-1-AP

Synonyms

F box only protein 22, F box protein 22, F box protein FBX22p44, FBX22, FBXO22, FISTC1

---

Validation Data Gallery

• 

  WB analysis using 13606-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13606-1-AP (FBXO22 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HuH-7 using 13606-1-AP

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 13606-1-AP (FBXO22 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 13606-1-AP

  IP result of anti-FBXO22 (IP:13606-1-AP, 4ug; Detection:13606-1-AP 1:4000) with HeLa cells lysate 1750 ug.

• 

  IHC staining of mouse heart using 13606-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 13606-1-AP (FBXO22 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 13606-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 13606-1-AP (FBXO22 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: HeLa cells, HuH-7 cells, HepG2 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 13 publications below
• WB: See 26 publications below
• IHC: See 11 publications below
• IF: See 2 publications below
• IP: See 3 publications below

Product Information

13606-1-AP targets FBXO22 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FBXO22 fusion protein Ag4485
• Full Name: F-box protein 22
• Calculated Molecular Weight: 403 aa, 45 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC039024
• Gene Symbol: FBXO22
• Gene ID (NCBI): 26263
• RRID: AB_2104403
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FBXO22, also named as FBX22, is a substrate-recognition of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. FBXO22 is a key regulator of senescence induction through ubiquitylation of p53 for degradation. FBXO22 also acts as a molecular switch for the antagonistic and agonistic actions of selective estrogen receptor modulators (SERM) and determines the sensitivity of breast cancer to SERM by ubiquitylating KDM4B complexed with unliganded or SERMs‐bound estrogen receptor (ER). (PMID: 32536008, PMID: 32249768)

Publications

Species	Application	Title
human	WB,IHC	Cell; Nrf2 Activation Promotes Lung Cancer Metastasis by Inhibiting the Degradation of Bach1.; Authors - Luca Lignitto ; KO Validated
human	WB	Nat Commun; Targeted inhibition of the COP9 signalosome for treatment of cancer.; Authors - Anita Schlierf
human	WB	Cell Death Differ; Global identification of phospho-dependent SCF substrates reveals a FBXO22 phosphodegron and an ERK-FBXO22-BAG3 axis in tumorigenesis.; Authors - Ping Liu ; KO Validated
human	WB,IHC,IF	Cell Death Dis; Targeting FBXO22 enhances radiosensitivity in non-small cell lung cancer by inhibiting the FOXM1/Rad51 axis; Authors - Yunshang Chen ; KD Validated
human	WB	Front Oncol; Pan-Cancer Analyses Reveal Oncogenic Role and Prognostic Value of F-Box Only Protein 22.; Authors - Sen Chen
human	WB	iScience; A small-molecule Skp1 inhibitor elicits cell death by p53-dependent mechanism.; Authors - Muzammal Hussain

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

CHAO (Verified Customer) (03-31-2022)

Strong single band at predicted size.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse Heart