FBXO2 Polyclonal antibody

• Featured Product
• KD/KO Validated

FBXO2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 14590-1-AP

Synonyms

F box only protein 2, F box protein 2, FBG1, Fbs1, FBX2, FBXO2, NFB42

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Validation Data Gallery

• 

  WB analysis using 14590-1-AP

  SH-SY5Y lysates were subjected to SDS PAGE followed by western blot with 14590-1-AP (FBXO2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 14590-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14590-1-AP (FBXO2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 14590-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14590-1-AP (FBXO2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 14590-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain using 14590-1-AP (FBXO2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of rat brain using 14590-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain using 14590-1-AP (FBXO2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of rat cerebellum using 14590-1-AP

  Immunohistochemical analysis of paraffin-embedded rat cerebellum using 14590-1-AP (FBXO2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of rat cerebellum using 14590-1-AP

  Immunohistochemical analysis of paraffin-embedded rat cerebellum using 14590-1-AP (FBXO2 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: SH-SY5Y cells
• Positive IHC detected in: mouse brain tissue, rat brain tissue, rat cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 4 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

14590-1-AP targets FBXO2 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FBXO2 fusion protein Ag6122
• Full Name: F-box protein 2
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 33-40 kDa
• GenBank Accession Number: BC025233
• Gene Symbol: FBXO2
• Gene ID (NCBI): 26232
• RRID: AB_2104394
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Sci Rep; Quantitative proteomics by SWATH-MS reveals sophisticated metabolic reprogramming in hepatocellular carcinoma tissues.; Authors - Yanyan Gao
human	WB,IHC	Biochem Biophys Res Commun; FBXO2, a novel marker for metastasis in human gastric cancer.; Authors - Sun Xu
human	WB	Front Med (Lausanne); Follicular dendritic cell differentiation is associated with distinct synovial pathotype signatures in rheumatoid arthritis; Authors - Mohey Eldin M El Shikh ; KD Validated
mouse	WB,IHC,CoIP	Nat Commun; Inhibition of histone methyltransferase Smyd3 rescues NMDAR and cognitive deficits in a tauopathy mouse model; Authors - Jamal B Williams
human,mouse	WB,IHC,IF,IP	World J Gastroenterol; F-box only protein 2 exacerbates non-alcoholic fatty liver disease by targeting the hydroxyl CoA dehydrogenase alpha subunit; Authors - Zhi Liu ; KD Validated
mouse	IF	Stem Cells; Early Wnt Signaling Activation Promotes Inner Ear Differentiation via Cell Caudalization in Mouse Stem Cell-Derived Organoids; Authors - Pei-Ciao Tang

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