FBXO18 Polyclonal antibody

FBXO18 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 14275-1-AP

Synonyms

FBX18, F-box only protein 18, F-box DNA helicase 1, FBH1, EC:5.6.2.4

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Validation Data Gallery

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  WB analysis of PC-3 using 14275-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 14275-1-AP (FBXO18 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14275-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14275-1-AP (FBXO18 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14275-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14275-1-AP (FBXO18 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF Staining of PC-3 using 14275-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed PC-3 cells using 14275-1-AP (FBXO18 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: PC-3 cells, HeLa cells
• Positive IF/ICC detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14275-1-AP targets FBXO18 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FBXO18 fusion protein Ag5622
• Full Name: F-box protein, helicase, 18
• Calculated Molecular Weight: 118 kDa, 123 kDa
• Observed Molecular Weight: 118-123 kDa
• GenBank Accession Number: BC032674
• Gene Symbol: FBXO18
• Gene ID (NCBI): 84893
• RRID: AB_10638916
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.