FBXL4 Polyclonal antibody

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  WB analysis using 24754-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24754-1-AP (FBXL4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of U2OS using 24754-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using FBXL4 antibody (24754-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

FBXL4 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 24754-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

FBL5, FBL4, F box/LRR repeat protein 4, F box protein FBL4/FBL5

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, mouse heart tissue, rat heart tissue
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

24754-1-AP targets FBXL4 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FBXL4 fusion protein Ag21579
• Full Name: F-box and leucine-rich repeat protein 4
• Calculated Molecular Weight: 621 aa, 70 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC055010
• Gene Symbol: FBXL4
• Gene ID (NCBI): 26235
• RRID: AB_3669448
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UKA2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FBXL4 (F-box and leucine-rich repeat protein 4) is a mitochondrial protein that has been recently found to play significant roles in mtDNA maintenance (PMID: 28940506). Moreover, FBXL4 is a substrate receptor of a Cullin-1-RING E3 (CRL1) ligase complex that has previously been shown to bind to SKP1, the common CRL1 adapter subunit (PMID: 37102372).