FBP2 Recombinant antibody, PBS Only

FBP2 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240879C9

Cat no : 83910-5-PBS

Synonyms

Muscle FBPase, Fructose-1,6-bisphosphatase isozyme 2, FBPase 2, EC:3.1.3.11, D-fructose-1,6-bisphosphate 1-phosphohydrolase 2

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Validation Data Gallery

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  WB analysis of mouse heart using 83910-5-RR (same clone as 83910-5-PBS)

  Mouse heart tissue were subjected to SDS PAGE followed by western blot with 83910-5-RR (FBP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83910-5-PBS in a different storage buffer formulation.

• 

  WB analysis of rat skeletal muscle using 83910-5-RR (same clone as 83910-5-PBS)

  Rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 83910-5-RR (FBP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83910-5-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 83910-5-RR (same clone as 83910-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 83910-5-RR (FBP2 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83910-5-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 83910-5-RR (same clone as 83910-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 83910-5-RR (FBP2 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83910-5-PBS in a different storage buffer formulation.

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  IF Staining of mouse skeletal muscle using 83910-5-RR (same clone as 83910-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using FBP2 antibody (83910-5-RR, Clone: 240879C9 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83910-5-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 83910-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83910-5-RR against Human FBP2 were performed. The affinity constant is 4.67 nM.

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Product Information

The immunogen of 83910-5-PBS is FBP2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: FBP2 fusion protein Ag18245
• Full Name: fructose-1,6-bisphosphatase 2
• Calculated Molecular Weight: 339 aa, 37 kDa
• Observed Molecular Weight: 37 kDa
• GenBank Accession Number: BC113632
• Gene Symbol: FBP2
• Gene ID (NCBI): 8789
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

FBP2, also named muscle FBP, belongs to the FBPase class 1 family. FBP2 is a ubiquitously expressed enzyme of glycogen synthesis from non-carbohydrates, e.g., lactate (glyconeogenesis). However, it also plays a variety of non-enzymatic functions. FBP2 is involved in the regulation of hypoxia-inducible factor 1 (Hif1) stability, cell cycle-dependent events, biogenesis of mitochondria, and protection of the organelles against high reactive oxygen species (ROS)- and high Ca2+-induced stress (PMID: 32962293, PMID: 35626746). The calculated molecular weight of FBP2 is 37 kDa.