FBP17 Polyclonal antibody

FBP17 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Reviews (2)

Cat no : 27056-1-AP

Synonyms

FBP17, FNBP1, formin binding protein 1, Formin binding protein 17, hFBP17, KIAA0554

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Validation Data Gallery

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  WB analysis of mouse brain using 27056-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 27056-1-AP (FBP17 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 27056-1-AP

  IP result of anti-FBP17 (IP:27056-1-AP, 4ug; Detection:27056-1-AP 1:1000) with mouse brain tissue lysate 3000ug.

• 

  IHC staining of human lung cancer using 27056-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27056-1-AP (FBP17 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human lung cancer using 27056-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27056-1-AP (FBP17 antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human breast cancer using 27056-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27056-1-AP (FBP17 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 27056-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27056-1-AP (FBP17 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 27056-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27056-1-AP (FBP17 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 27056-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27056-1-AP (FBP17 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 27056-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27056-1-AP (FBP17 antibody) at dilution of 1:50 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 27056-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27056-1-AP (FBP17 antibody) at dilution of 1:50 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human lung cancer tissue, human breast cancer tissue, mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:150-1:600
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 27056-1-AP is FBP17 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FBP17 fusion protein Ag25784
• Full Name: formin binding protein 1
• Observed Molecular Weight: 80-85 kDa
• GenBank Accession Number: BC101755
• Gene Symbol: FBP17
• Gene ID (NCBI): 23048
• RRID: AB_2880734
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FBP17 (formin-binding protein 17) is a new dynamin-associating molecule consisting of several functional domains, including an N-terminal EFC domain and an SH3 domain at the C-terminus. FBP17 is involved in dynamin-mediated endocytosis and has been reported to be required for invadopodia formation and tumor cell invasion.

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Cicek (Verified Customer) (06-11-2024)

I tested the antibody on mouse neurons, iPSCs and 3-weeks-old iNeurons with Western blotting using beta-actin as positive control. There were bands at the 80 kDa mark showing FBP17 for iPSC and iNeurons, but not mouse neurons. All samples showed a strong unspecific band at 70 kDa mark.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000

FH

María (Verified Customer) (02-08-2022)

Works fine for WB (1:1000), for IF some background, even after several washes with detergent

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Hela/RPE-1/MEF/NaTi