Various lysates were subjected to SDS PAGE followed by western blot with 68446-1-Ig (FBP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 68446-1-Ig (FBP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 68446-1-Ig
MCF-7 cells were subjected to SDS PAGE followed by western blot with 68446-1-Ig (FBP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
MCF-7 cells were subjected to SDS PAGE followed by western blot with 68446-1-Ig (FBP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis of T-47D using 68446-1-Ig
T-47D cells were subjected to SDS PAGE followed by western blot with 68446-1-Ig (FBP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
T-47D cells were subjected to SDS PAGE followed by western blot with 68446-1-Ig (FBP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis of pig liver using 68446-1-Ig
pig liver tissue were subjected to SDS PAGE followed by western blot with 68446-1-Ig (FBP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
pig liver tissue were subjected to SDS PAGE followed by western blot with 68446-1-Ig (FBP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
IHC staining of mouse kidney using 68446-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68446-1-Ig (FBP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68446-1-Ig (FBP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of MCF-7 using 68446-1-Ig
Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using FBP1 antibody (68446-1-Ig, Clone: 2G4E4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).
Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using FBP1 antibody (68446-1-Ig, Clone: 2G4E4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).
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mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
MCF-7 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:5000-1:40000
Immunohistochemistry (IHC)
IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICC
IF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
68446-1-Ig targets FBP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
FBP1 (Fructose-1,6-bisphosphatase 1) is also named as FBP and belongs to the FBPase class 1 family. It catalyzes the hydrolysis of fructose-1,6 bisphosphate to fructose-6-phosphate and inorganic phosphate. This reaction is an important regulatory site of gluconeogenesis. Defects in FBP1 are the cause of fructose-1,6-bisphosphatase deficiency (FBPD) (PMID:12126934).
Various lysates were subjected to SDS PAGE followed by western blot with 68446-1-Ig (FBP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 68446-1-Ig
MCF-7 cells were subjected to SDS PAGE followed by western blot with 68446-1-Ig (FBP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis of T-47D using 68446-1-Ig
T-47D cells were subjected to SDS PAGE followed by western blot with 68446-1-Ig (FBP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis of pig liver using 68446-1-Ig
pig liver tissue were subjected to SDS PAGE followed by western blot with 68446-1-Ig (FBP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of mouse kidney using 68446-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68446-1-Ig (FBP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 68446-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68446-1-Ig (FBP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF/ICC Figures
IF Staining of MCF-7 using 68446-1-Ig
Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using FBP1 antibody (68446-1-Ig, Clone: 2G4E4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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