fibrillin 2-Specific Polyclonal antibody

fibrillin 2-Specific Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 20252-1-AP

Synonyms

CCA, DA9, FBN2, fibrillin 2, fibrillin 2-Specific

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Validation Data Gallery

• 

  WB analysis of human brain using 20252-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 20252-1-AP (fibrillin 2-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 20252-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 20252-1-AP (fibrillin 2-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 20252-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20252-1-AP (fibrillin 2-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 20252-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20252-1-AP (fibrillin 2-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 20252-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 20252-1-AP (fibrillin 2-Specific antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 20252-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 20252-1-AP (fibrillin 2-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 20252-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 20252-1-AP (fibrillin 2-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human placenta using 20252-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20252-1-AP (fibrillin 2-Specific antibody at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human placenta using 20252-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20252-1-AP (fibrillin 2-Specific antibody at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human placenta using 20252-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20252-1-AP (fibrillin 2-Specific antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human placenta using 20252-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20252-1-AP (fibrillin 2-Specific antibody at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: human brain tissue, HepG2 cells, L02 cells, mouse lung tissue
• Positive IHC detected in: human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

20252-1-AP targets fibrillin 2-Specific in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: fibrillin 2
• Calculated Molecular Weight: 315 kDa
• Observed Molecular Weight: 290 kDa, 160 kDa
• GenBank Accession Number: NM_001999
• Gene Symbol: Fibrillin 2
• Gene ID (NCBI): 2201
• RRID: AB_11182265
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FBN2 belongs to the fibrillin family. Fibrillins are structural components of 10-12 nm extracellular calcium-binding microfibrils which occur either in association with elastin or in elastin-free bundles. Fibrillin-2-containing microfibrils regulate the early process of elastic fiber assembly. Defects in FBN2 are the cause of congenital contractural arachnodactyly (CCA) which also known as Beals syndrome or distal arthrogryposis type 9 (DA9). This antibody is specific to FBN2. It recognizes both two isoforms ~300 kDa and 160 kDa.

Publications

Species	Application	Title
human	WB	Head Neck; High expression of NFX1-123 in HPV positive head and neck squamous cell carcinomas.; Authors - Sreenivasulu Chintala
mouse	IHC	J Ovarian Res; hUMSC transplantation restores follicle development in ovary damaged mice via re-establish extracellular matrix (ECM) components; Authors - Yin Shuyuan