Fibrillin 1 Recombinant antibody, PBS Only (Capture)

Fibrillin 1 Uni-rAb^TM Recombinant Antibody for IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240960E11

Cat no : 83836-2-PBS

Synonyms

FBN1, MASS, Fibrillin-1, FBN, Asprosin

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Validation Data Gallery

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  Sandwich ELISA standard curve of MP00807-1

  Sandwich ELISA standard curve of MP00807-1, Human Fibrillin 1/FBN1 Recombinant Matched Antibody Pair - PBS only. 83836-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag30283. 83836-1-PBS was HRP conjugated as the detection antibody. Range: 62.5-4000 pg/mL

• 

  Sample test of MP00807-1

  Serum of sixteen individual healthy human donors was measured. The human Fibrillin 1/FBN1 concentration of detected samples was determined to be 117.73 ng/mL with a range of 43.48 - 246.44 ng/mL.

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  IF Staining of HeLa using 83836-2-RR (same clone as 83836-2-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Fibrillin 1 antibody (83836-2-RR, Clone: 240960E11 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83836-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00807-1

  Cytometric bead array standard curve of MP00807-1, Fibrillin 1/FBN1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83836-2-PBS. Detection antibody: 83836-1-PBS. Standard: Ag30283. Range: 0.313-40 ng/mL.

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  Affinity and Kinetic Characterization of 83836-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83836-2-RR against Human Fibrillin 1 were performed. The affinity constant is 0.103 nM.

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Product Information

83836-2-PBS targets Fibrillin 1 as part of a matched antibody pair:

MP00807-1: 83836-2-PBS capture and 83836-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fibrillin 1 fusion protein Ag30283
• Full Name: fibrillin 1
• GenBank Accession Number: BC146854
• Gene Symbol: Fibrillin 1
• Gene ID (NCBI): 2200
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Fibrillin-1 belongs to the fibrillin family. Fibrillins are structural components of 10-12 nm extracellular calcium-binding microfibrils, which occur either in association with elastin or in elastin-free bundles. Fibrillin-1-containing microfibrils provide long-term force bearing structural support. Defects in Fibrillin-1 are a cause of Marfan syndrome (MFS), isolated ectopia lentis (EL), Weill-Marchesani syndrome autosomal dominant (ADWMS), Shprintzen-Goldberg craniosynostosis syndrome (SGS) or MASS syndrome.