FATP5/SLC27A5 Polyclonal antibody

FATP5/SLC27A5 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 25347-1-AP

Synonyms

FATP5, SLC27A5, Cholate--CoA ligase, Bile acyl-CoA synthetase, Bile acid-CoA ligase

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Validation Data Gallery

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  WB analysis using 25347-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25347-1-AP (FATP5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of rat liver using 25347-1-AP

  IP result of anti-FATP5 (IP:25347-1-AP, 4ug; Detection:25347-1-AP 1:5000) with rat liver tissue lysate 2695 ug.

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Tested Applications

• Positive WB detected in: mouse liver tissue, rat liver tissue
• Positive IP detected in: rat liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

25347-1-AP targets FATP5/SLC27A5 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FATP5/SLC27A5 fusion protein Ag18446
• Full Name: solute carrier family 27 (fatty acid transporter), member 5
• Calculated Molecular Weight: 690 aa, 75 kDa
• Observed Molecular Weight: 70-75 kDa
• GenBank Accession Number: BC148807
• Gene Symbol: FATP5/SLC27A5
• Gene ID (NCBI): 10998
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC27A5, also known as FATP5, ACSVL6, and BAL, is an isozyme of very long-chain acyl-CoA synthetase (VLCS). It is capable of activating very long-chain fatty acids containing 24- and 26-carbons(PMID: 10479480). FATP5 mediates the import of long-chain fatty acids (LCFA) by facilitating their transport across cell membranes (PubMed:20448275). FATP5 is associated with endoplasmic reticulum but not with peroxisomes, and its primary role is in fatty acid elongation or complex lipid synthesis. FATP5 is exclusively expressed by the liver and localized to the basal plasma membrane of hepatocytes (PMID:16618416).

Publications

Species	Application	Title
human	WB,IHC	Front Oncol; FATP5 modulates biological activity and lipid metabolism in prostate cancer through the TEAD4-mediated Hippo signaling; Authors - Shenyang Liu