FATP2 Monoclonal antibody, PBS Only

FATP2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2C10D7

Cat no : 68074-1-PBS

Synonyms

SLC27A2, EC:6.2.1.24, EC:6.2.1.15, EC:6.2.1.-, Arachidonate--CoA ligase

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Validation Data Gallery

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  WB analysis of rat liver using 68074-1-Ig (same clone as 68074-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 68074-1-Ig (FATP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68074-1-PBS in a different storage buffer formulation.

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  WB analysis using 68074-1-Ig (same clone as 68074-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68074-1-Ig (SLC27A2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68074-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 68074-1-Ig (same clone as 68074-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 68074-1-Ig (SLC27A2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68074-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 68074-1-Ig (same clone as 68074-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68074-1-Ig (FATP2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68074-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 68074-1-Ig (same clone as 68074-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68074-1-Ig (FATP2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68074-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 68074-1-Ig (same clone as 68074-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FATP2 antibody (68074-1-Ig, Clone: 2C10D7 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 68074-1-PBS in a different storage buffer formulation.

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Product Information

68074-1-PBS targets FATP2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: FATP2 fusion protein Ag5390
• Full Name: solute carrier family 27 (fatty acid transporter), member 2
• Calculated Molecular Weight: 567 aa, 65 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC057770
• Gene Symbol: FATP2/SLC27A2
• Gene ID (NCBI): 11001
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

FATP2 is a member of the FATP family which functions in lipid and bile metabolism. It is a 70-kDa protein predominantly expressed in liver and kidney. Kidney FATP2 is localized exclusively to proximal tubule epithelial cells along the apical but not the basolateral membrane, and regulates lipoapoptosis. FATP2 is involved in metabolism-related diseases including nonalcoholic fatty liver disease (NAFLD) and type 2 diabetes mellitus (T2DM), and is a potential clinical biomarker and therapeutic target.