FAT2 Polyclonal antibody

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  WB analysis using 31490-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31490-1-AP (FAT2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

FAT2 Polyclonal Antibody for WB, ELISA

Cat No. 31490-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Protocadherin Fat 2, MEGF1, KIAA0811, HFAT2, CDHF8

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Tested Applications

• Positive WB detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31490-1-AP targets FAT2 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FAT2 fusion protein Ag34942
• Full Name: FAT tumor suppressor homolog 2 (Drosophila)
• Calculated Molecular Weight: 479 kDa
• Observed Molecular Weight: 500-600 kDa
• GenBank Accession Number: NM_001447
• Gene Symbol: FAT2
• Gene ID (NCBI): 2196
• RRID: AB_3670004
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q9NYQ8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FAT2 (FAT atypical cadherin 2), also known as CDHF8. The gene product is a member of the cadherin superfamily, a group of integral membrane proteins characterized by the presence of cadherin-type repeats. This protein most likely functions as a cell adhesion molecule, controlling cell proliferation and playing an important role in cerebellum development. Expressed in epidermal keratinocytes, infant brain, cerebellum, and also in a variety of tumors, such as pancreatic cancer, diffuse type gastric cancer, ovarian cancer, esophageal cancer, skin squamous cell carcinoma, head and neck cancer. Not expressed in melanoma cell line A375 cells, normal epidermal melanocytes or normal dermal fibroblasts. Expressed in epidermal keratinocytes and squamous cell carcinoma (at protein level). The molecular weight of FAT2 is 473 kDa. The huge molecular masses (about 500-600 kDa) have hindered studies of the molecular aspects of itself roles(PMID: 15923647).