FAT10 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

FAT10 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 13003-2-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 13003-2-AP (same clone as 13003-2-PBS)

  TNF alpha and IFN gamma treated HepG2 cells were subjected to SDS PAGE followed by western blot with 13003-2-AP (FAT10 antibody) at dilution of 1:1000 and incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13003-2-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 13003-2-AP (same clone as 13003-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13003-2-AP (FAT10 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13003-2-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 13003-2-AP (same clone as 13003-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13003-2-AP (FAT10 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13003-2-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 13003-2-AP (same clone as 13003-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13003-2-AP (FAT10 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13003-2-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 13003-2-AP (same clone as 13003-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13003-2-AP (FAT10 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13003-2-PBS in a different storage buffer formulation.

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Product Information

13003-2-PBS targets FAT10 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FAT10 fusion protein Ag3680
• Full Name: ubiquitin D
• Calculated Molecular Weight: 165 aa, 18 kDa
• Observed Molecular Weight: 20 kDa, 70kDa
• GenBank Accession Number: BC012472
• Gene Symbol: FAT10
• Gene ID (NCBI): 10537
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

FAT10, also named UBD, contains two ubiquitin-like domains. It is a ubiquitin-like protein modifier that can be covalently attached to the target protein and subsequently leads to their degradation by the 26S proteasome, in a NUB1L-dependent manner. FAT10 also has important roles in cell mitosis, chromosome instability, apoptosis, and immune response. FAT10 mediates apoptosis in a caspase-dependent manner, especially in the renal epithelium and tubular cells during renal diseases such as polycystic kidney disease and Human immunodeficiency virus (HIV)-associated nephropathy (HIVAN). It promotes the expression of the proteasome subunit beta type-9 (PSMB9/LMP2). FAT10 regulates TNF-alpha-induced and LPS-mediated activation of the central mediator of innate immunity NF-kappa-B by promoting TNF-alpha-mediated proteasomal degradation of ubiquitinated-I-kappa-B-alpha. It may be involved in dendritic cell (DC) maturation, the process by which immature dendritic cells differentiate into fully competent antigen-presenting cells that initiate T-cell responses. FAT10 may be a marker for precancerous lesions and may promote cancer progression. This antibody is a rabbit polyclonal antibody raised against full-length FAT10 of human origin.