FASTKD2 Polyclonal antibody

• Featured Product
• KD/KO Validated

FASTKD2 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, RIP, ELISA

Conjugate

Unconjugated

Publications

19

Reviews (1)

Cat no : 17464-1-AP

Synonyms

FASTKD 2, FAST kinase domain-containing protein 2, mitochondrial

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Validation Data Gallery

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  WB analysis using 17464-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17464-1-AP (FASTKD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 17464-1-AP

  WB result of FASTKD2 antibody (17464-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FASTKD2 transfected HepG2 cells.

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  IP experiment of HepG2 using 17464-1-AP

  IP result of anti-FASTKD2 (IP:17464-1-AP, 4ug; Detection:17464-1-AP 1:1000) with HepG2 cells lysate 2040 ug.

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  IF Staining of COS7 cells using 17464-1-AP

  IF result of anti-FASTKD2 (17464-1-AP) with COS7 cells.

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  IF Staining of U2OS using 17464-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using FASTKD2 antibody (17464-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of COS7 cells using 17464-1-AP

  IF result of anti- FASTKD2 (17464-1-AP) with FASTKD2-YFP overexpression in COS7 cells.

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  IF Staining of U2OS cells using 17464-1-AP

  IF result of anti-FASTKD2 (17464-1-AP) with U2OS cells.

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, K-562 cells
• Positive IP detected in: HepG2 cells
• Positive IF/ICC detected in: COS7 cells, U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 11 publications below
• IHC: See 1 publications below
• IF: See 9 publications below
• RIP: See 1 publications below

Product Information

17464-1-AP targets FASTKD2 in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FASTKD2 fusion protein Ag11314
• Full Name: FAST kinase domains 2
• Calculated Molecular Weight: 710 aa, 81 kDa
• Observed Molecular Weight: 55-65 kDa
• GenBank Accession Number: BC001544
• Gene Symbol: FASTKD2
• Gene ID (NCBI): 22868
• RRID: AB_2101119
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FASTKD2 encodes a FAS-activated serine-threonine (FAST) kinase domain 2 which locates in the mitochondrial inner compartment. FASTKD2 is a member of the human FASTK protein family, formed by six architecturally related proteins named FASTK and FASTKD1-5 that localize to mitochondria. The predicted MW of FASTKD2 is 81 kDa, yet the western blot of FASTKD2 revealed a band of ~60 kDa. (PMID: 26370583, PMID: 26990993, PMID: 25683715)

Publications

Species	Application	Title
human	IF,WB	Nat Cell Biol; Mitochondrial RNA granules are fluid condensates positioned by membrane dynamics.; Authors - Timo Rey
human	WB	Cell Metab; A Genome-wide CRISPR Death Screen Identifies Genes Essential for Oxidative Phosphorylation.; Authors - Jason D Arroyo
human	IF	Nucleic Acids Res; FASTKD1 and FASTKD4 have opposite effects on expression of specific mitochondrial RNAs, depending upon their endonuclease-like RAP domain.; Authors - Erik Boehm
human	WB	EMBO Rep; A pseudouridine synthase module is essential for mitochondrial protein synthesis and cell viability.; Authors - Hana Antonicka
human	WB, IF, RIP	Cell Rep; Mitochondrial RNA Granules Are Centers for Posttranscriptional RNA Processing and Ribosome Biogenesis.; Authors - Hana Antonicka ; KD Validated
	IF	Cell Rep; A mitochondria-specific isoform of FASTK is present in mitochondrial RNA granules and regulates gene expression and function.; Authors - Alexis A Jourdain

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Erik (Verified Customer) (02-25-2021)

We used the antibody at 1:500 dilution, and it produced two clean bands for the two protein isoforms annotated in UniProt, at the expected height

• Applications: Western Blot
• Cell Tissue Type: PANC1 and MIAPACA2 cell lines

FASTKD2 Antibody Western Blot validation ( dilution) in PANC1 and MIAPACA2 cell lines (Cat no:17464-1-AP)