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FASTKD2 Polyclonal antibody
FASTKD2 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, IF/ICC, IP, RIP, ELISA
Conjugate
Unconjugated
Cat no : 17464-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, HepG2 cells, K-562 cells |
Positive IP detected in | HepG2 cells |
Positive IF/ICC detected in | COS7 cells, U2OS cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 4 publications below |
WB | See 11 publications below |
IHC | See 1 publications below |
IF | See 9 publications below |
RIP | See 1 publications below |
Product Information
17464-1-AP targets FASTKD2 in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, zebrafish |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | FASTKD2 fusion protein Ag11314 |
Full Name | FAST kinase domains 2 |
Calculated Molecular Weight | 710 aa, 81 kDa |
Observed Molecular Weight | 55-65 kDa |
GenBank Accession Number | BC001544 |
Gene Symbol | FASTKD2 |
Gene ID (NCBI) | 22868 |
RRID | AB_2101119 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
FASTKD2 encodes a FAS-activated serine-threonine (FAST) kinase domain 2 which locates in the mitochondrial inner compartment. FASTKD2 is a member of the human FASTK protein family, formed by six architecturally related proteins named FASTK and FASTKD1-5 that localize to mitochondria. The predicted MW of FASTKD2 is 81 kDa, yet the western blot of FASTKD2 revealed a band of ~60 kDa. (PMID: 26370583, PMID: 26990993, PMID: 25683715)
Protocols
Product Specific Protocols | |
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WB protocol for FASTKD2 antibody 17464-1-AP | Download protocol |
IF protocol for FASTKD2 antibody 17464-1-AP | Download protocol |
IP protocol for FASTKD2 antibody 17464-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Cell Biol Mitochondrial RNA granules are fluid condensates positioned by membrane dynamics. | ||
Cell Metab A Genome-wide CRISPR Death Screen Identifies Genes Essential for Oxidative Phosphorylation. | ||
Nucleic Acids Res FASTKD1 and FASTKD4 have opposite effects on expression of specific mitochondrial RNAs, depending upon their endonuclease-like RAP domain. | ||
EMBO Rep A pseudouridine synthase module is essential for mitochondrial protein synthesis and cell viability. | ||
Cell Rep Mitochondrial RNA Granules Are Centers for Posttranscriptional RNA Processing and Ribosome Biogenesis.
| ||
Cell Rep A mitochondria-specific isoform of FASTK is present in mitochondrial RNA granules and regulates gene expression and function. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Erik (Verified Customer) (02-25-2021) | We used the antibody at 1:500 dilution, and it produced two clean bands for the two protein isoforms annotated in UniProt, at the expected height
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