FARSB Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

FARSB Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1H10C2

Cat no : 67924-1-PBS

Synonyms

FARSB, FARSLB, FRSB, HSPC173, PheHB, PheRS

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Validation Data Gallery

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  WB analysis using 67924-1-Ig (same clone as 67924-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67924-1-Ig (FARSB antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67924-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 67924-1-Ig (same clone as 67924-1-PBS)

  WB result of FARSB antibody (67924-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FARSB transfected HepG2 cells. This data was developed using the same antibody clone with 67924-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 67924-1-Ig (same clone as 67924-1-PBS)

  WB result of FARSB antibody (67924-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FARSB transfected HeLa cells. This data was developed using the same antibody clone with 67924-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 67924-1-Ig (same clone as 67924-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67924-1-Ig (FARSB antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67924-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67924-1-Ig (same clone as 67924-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67924-1-Ig (FARSB antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67924-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67924-1-Ig (same clone as 67924-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67924-1-Ig (FARSB antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67924-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 67924-1-Ig (same clone as 67924-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67924-1-Ig (FARSB antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67924-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 67924-1-Ig (same clone as 67924-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67924-1-Ig (FARSB antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67924-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 67924-1-Ig (same clone as 67924-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67924-1-Ig (FARSB antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67924-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 67924-1-Ig (same clone as 67924-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67924-1-Ig (FARSB antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67924-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 67924-1-Ig (same clone as 67924-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67924-1-Ig (FARSB antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67924-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 67924-1-Ig (same clone as 67924-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67924-1-Ig (FARSB antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67924-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 67924-1-Ig (same clone as 67924-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using FARSB antibody (67924-1-Ig, Clone: 1H10C2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67924-1-PBS in a different storage buffer formulation.

• 

  IF Staining of U-251 using 67924-1-Ig (same clone as 67924-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed U-251 cells using FARSB antibody (67924-1-Ig, Clone: 1H10C2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67924-1-PBS in a different storage buffer formulation.

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Product Information

67924-1-PBS targets FARSB in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: FARSB fusion protein Ag9695
• Full Name: phenylalanyl-tRNA synthetase, beta subunit
• Calculated Molecular Weight: 589 aa, 66 kDa
• Observed Molecular Weight: 66 kDa
• GenBank Accession Number: BC017783
• Gene Symbol: FARSB
• Gene ID (NCBI): 10056
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.