FARSB Polyclonal antibody

FARSB Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 16341-1-AP

Synonyms

Phenylalanine--tRNA ligase beta subunit, PheHB, HSPC173, FRSB, FARSLB

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Validation Data Gallery

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  WB analysis using 16341-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16341-1-AP (FARSB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 16341-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16341-1-AP (FARSB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 16341-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 16341-1-AP (FARSB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 16341-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 16341-1-AP (FARSB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16341-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16341-1-AP (FARSB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 16341-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16341-1-AP (FARSB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 16341-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 16341-1-AP (FARSB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 16341-1-AP

  IP result of anti-FARSB (IP:16341-1-AP, 4ug; Detection:16341-1-AP 1:500) with HeLa cells lysate 4000ug.

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  IHC staining of mouse brain using 16341-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16341-1-AP (FARSB antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 16341-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16341-1-AP (FARSB antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 16341-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 16341-1-AP (FARSB antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 16341-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 16341-1-AP (FARSB antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 16341-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 16341-1-AP (FARSB antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 16341-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 16341-1-AP (FARSB antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 16341-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16341-1-AP (FARSB antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 16341-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16341-1-AP (FARSB antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 16341-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 16341-1-AP (FARSB antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 16341-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 16341-1-AP (FARSB antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 16341-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 16341-1-AP (FARSB antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells, HL-60 cells, K-562 cells, human liver tissue, mouse brain tissue, PC-3 cells, mouse liver tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: mouse brain tissue, mouse cerebellum tissue, human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Product Information

16341-1-AP targets FARSB in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FARSB fusion protein Ag9522
• Full Name: phenylalanyl-tRNA synthetase, beta subunit
• Calculated Molecular Weight: 589 aa, 66 kDa
• Observed Molecular Weight: 66 kDa
• GenBank Accession Number: BC017783
• Gene Symbol: FARSB
• Gene ID (NCBI): 10056
• RRID: AB_2102515
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
		J Am Chem Soc; Oxidation-Controlled, Strain-Promoted Tellurophene-Alkyne Cycloaddition (OSTAC): A Bioorthogonal Tellurophene-Dependent Conjugation Reaction; Authors - Yong Jia Bu
human,rat	WB	Nat Commun; Cis- and trans-resveratrol have opposite effects on histone serine-ADP-ribosylation and tyrosine induced neurodegeneration.; Authors - Megha Jhanji
rat	WB	Neural Regen Res; A novel therapeutic target for peripheral nerve injury-related diseases: aminoacyl-tRNA synthetases.; Authors - Byung Sun Park
human,mouse	WB,IF	Nat Commun; Phenylalanine-tRNA aminoacylation is compromised by ALS/FTD-associated C9orf72 C4G2 repeat RNA; Authors - Mirjana Malnar Črnigoj
human,mouse	WB,IHC,IF	Int J Mol Sci; FARSB Facilitates Hepatocellular Carcinoma Progression by Activating the mTORC1 Signaling Pathway; Authors - Yaofeng Wang