FARSA Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

FARSA Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 18121-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 18121-1-AP (same clone as 18121-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 18121-1-AP (FARSA antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18121-1-PBS in a different storage buffer formulation.

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  WB analysis using 18121-1-AP (same clone as 18121-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 18121-1-AP (FARSA antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18121-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 18121-1-AP (same clone as 18121-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 18121-1-AP (FARSA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18121-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 18121-1-AP (same clone as 18121-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 18121-1-AP (FARSA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18121-1-PBS in a different storage buffer formulation.

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  IP experiment of MCF-7 using 18121-1-AP (same clone as 18121-1-PBS)

  IP result of anti-FARSA (IP:18121-1-AP, 4ug; Detection:18121-1-AP 1:2000) with MCF-7 cells lysate 3240 ug. This data was developed using the same antibody clone with 18121-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 18121-1-AP (same clone as 18121-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18121-1-AP (FARSA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18121-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 18121-1-AP (same clone as 18121-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18121-1-AP (FARSA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18121-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 18121-1-AP (same clone as 18121-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 18121-1-AP (FARSA antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 18121-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 18121-1-AP (same clone as 18121-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 18121-1-AP (FARSA antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 18121-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 18121-1-AP (same clone as 18121-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using FARSA antibody (18121-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 18121-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 18121-1-AP (same clone as 18121-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using FARSA antibody (18121-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 18121-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 18121-1-AP (same clone as 18121-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using FARSA antibody (18121-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 18121-1-PBS in a different storage buffer formulation.

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Product Information

18121-1-PBS targets FARSA in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FARSA fusion protein Ag12647
• Full Name: phenylalanyl-tRNA synthetase, alpha subunit
• Calculated Molecular Weight: 58 kDa
• Observed Molecular Weight: 57 kDa
• GenBank Accession Number: BC006495
• Gene Symbol: FARSA
• Gene ID (NCBI): 2193
• RRID: AB_2102509
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.