FAR2 Polyclonal antibody

FAR2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27717-1-AP

Synonyms

SDR10E2, MLSTD1, HEL-S-81

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Validation Data Gallery

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  WB analysis using 27717-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27717-1-AP (FAR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 27717-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27717-1-AP (FAR2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 27717-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27717-1-AP (FAR2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Neuro-2a cells, mouse brain tissue, rat brain tissue
• Positive IHC detected in: human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27717-1-AP targets FAR2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FAR2 fusion protein Ag26819
• Full Name: fatty acyl CoA reductase 2
• Calculated Molecular Weight: 515 aa, 59 kDa
• Observed Molecular Weight: 59 kDa
• GenBank Accession Number: BC022267
• Gene Symbol: FAR2
• Gene ID (NCBI): 55711
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fatty acyl-CoA reductase 2 (FAR2), also known as MLSTD1, belongs to the fatty acyl-CoA reductase family. FAR2 catalyzes the reduction of saturated but not unsaturated C16 or C18 fatty acyl-CoA to fatty alcohols (PMID: 24009241). FAR2 may play a role in the production of ether lipids/plasmalogens and wax monoesters which synthesis requires fatty alcohols as substrates. FAR2 has 2 isoforms with the molecular mass of 49 kDa and 59 kDa (Refer to Uniprot).