FANCL Polyclonal antibody

• Featured Product
• KD/KO Validated

FANCL Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14454-1-AP

Synonyms

FAAP43, FANCL, Fanconi anemia group L protein, PHF9, POG

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Validation Data Gallery

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  WB analysis of HEK-293 using 14454-1-AP

  WB result of FANCL antibody (14454-1-AP; 1:200; incubated at room temperature for 1.5 hours) with sh-Control and sh-FANCL transfected HEK-293 cells.

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  WB analysis of A549 using 14454-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 14454-1-AP (FANCL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 14454-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14454-1-AP (FANCL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 14454-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14454-1-AP (FANCL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 14454-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14454-1-AP (FANCL Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 14454-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14454-1-AP (FANCL Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: A549 cells, HEK-293 cells, HEK-cells, Hela cells, HepG2 cells
• Positive IHC detected in: human kidney tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14454-1-AP targets FANCL in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FANCL fusion protein Ag6031
• Full Name: Fanconi anemia, complementation group L
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC054517
• Gene Symbol: FANCL
• Gene ID (NCBI): 55120
• RRID: AB_10644333
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fanconi anemia (FA) is a hereditary bone marrow failure syndrome associated with developmental defects and cancer predisposition. The FA nuclear core complex consists of FANCA, B, C, E, F, G, L, and M. FANCL is a RING-type E3 ubiquitin ligase that monoubiquitinates FANCD2 and FANCI, a key functional role facilitated by other members of the nuclear core complex (PMID: 23783032). FANCL plays an important role in the repair of cisplatin-induced DNA damage (PMID: 26385482) and participates directly in support of stem cell function (PMID: 23783032). FANCL can be detected as 40-43 kDa (full-length) and ~28 kDa.