FANCI Monoclonal antibody

FANCI Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

3

CloneNo.

1B10E6

Cat no : 67304-1-Ig

Synonyms

Protein FACI, Fanconi anemia group I protein

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Validation Data Gallery

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  WB analysis using 67304-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67304-1-Ig (FANCI antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67304-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67304-1-Ig (FANCI antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67304-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67304-1-Ig (FANCI antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skin cancer using 67304-1-Ig

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 67304-1-Ig (FANCI antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 67304-1-Ig

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 67304-1-Ig (FANCI antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, A431 cells, HEK-293 cells, Jurkat cells, K-562 cells, LNCaP cells, A549 cells, Raji cells, Daudi cells
• Positive IHC detected in: human skin cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 1 publications below

Product Information

67304-1-Ig targets FANCI in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: FANCI fusion protein Ag15153
• Full Name: Fanconi anemia, complementation group I
• Calculated Molecular Weight: 1328 aa, 149 kDa
• Observed Molecular Weight: 149 kDa, 142 kDa
• GenBank Accession Number: BC004277
• Gene Symbol: FANCI
• Gene ID (NCBI): 55215
• RRID: AB_2882566
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Adv Sci (Weinh); HPV Enhances HNSCC Chemosensitization by Inhibiting SERPINB3 Expression to Disrupt the Fanconi Anemia Pathway; Authors - Zixian Huang
human	WB,IHC	Front Immunol; FANCI serve as a prognostic biomarker correlated with immune infiltrates in skin cutaneous melanoma; Authors - Zhenguo Cai
human	WB	Adv Sci (Weinh); The Compromised Fanconi Anemia Pathway in Prelamin A-Expressing Cells Contributes to Replication Stress-Induced Genomic Instability; Authors - Pengqing Nie