FANCI Polyclonal antibody

FANCI Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 20789-1-AP

Synonyms

Protein FACI, Fanconi anemia group I protein



Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, Jurkat cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20789-1-AP targets FANCI in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FANCI fusion protein Ag14386
Full Name Fanconi anemia, complementation group I
Calculated Molecular Weight 1328 aa, 149 kDa
Observed Molecular Weight 149 kDa
GenBank Accession NumberBC004277
Gene Symbol FANCI
Gene ID (NCBI) 55215
RRIDAB_10694829
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for FANCI antibody 20789-1-APDownload protocol
IHC protocol for FANCI antibody 20789-1-APDownload protocol
IP protocol for FANCI antibody 20789-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cancer

Aberrant R-loop-mediated immune evasion, cellular communication, and metabolic reprogramming affect cancer progression: a single-cell analysis

Authors - Shichao Zhang
humanWB

Sci Adv

Deficiency in mammalian STN1 promotes colon cancer development via inhibiting DNA repair

Authors - Dinh Duc Nguyen
humanWB,CoIP

Cell Death Dis

Inactivation of ribosomal protein S27-like impairs DNA interstrand cross-link repair by destabilization of FANCD2 and FANCI.

Authors - Siyuan Sun
human

Adv Sci (Weinh)

Phosphorylation of USP29 by CDK1 Governs TWIST1 Stability and Oncogenic Functions

Authors - Tangming Guan
WB

Mol Carcinog

KNTC1 knockdown inhibits the proliferation and migration of osteosarcoma cells by MCM2

Authors - Lei Zhong