FANCF Polyclonal antibody

FANCF Polyclonal Antibody for IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IP, ELISA

Conjugate

Unconjugated

Cat no : 18060-1-AP

Synonyms

FAF, FANCF, Fanconi anemia group F protein, Protein FACF

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Validation Data Gallery

• 

  IP experiment of HeLa using 18060-1-AP

  IP result of anti-FANCF (IP:18060-1-AP, 4ug; Detection:18060-1-AP 1:400) with HeLa cells lysate 1600 ug.

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Tested Applications

• Positive IP detected in: HeLa cells

Recommended dilution

• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

18060-1-AP targets FANCF in IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FANCF fusion protein Ag12649
• Full Name: Fanconi anemia, complementation group F
• Calculated Molecular Weight: 374 aa, 42 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC093867
• Gene Symbol: FANCF
• Gene ID (NCBI): 2188
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The Fanconi anemia complement group F (FANCF) is located on chromosome 11p15, a region enriched in cancer-associated genes. FANCF is known to be involved in DNA repair, and the overexpression of FANCF protein leads to cell proliferation and ultimately to cancer. The epigenetic silencing of FANCF has been reported in several different tumors such as ovaries, bladder, cervical, leukemic, testicular, lung, and oral tumors (PMID: 21915857, 32915143).