FANCA-Specific Polyclonal antibody

FANCA-Specific Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 19542-1-AP

Synonyms

FA, FA H, FA1, FAA, FACA, FAH, FANCA, FANCA-Specific, FANCH, Fanconi anemia group A protein, Protein FACA

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Validation Data Gallery

• 

  WB analysis of HeLa using 19542-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19542-1-AP (FANCA-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 19542-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 19542-1-AP (FANCA-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 19542-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19542-1-AP (FANCA-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

19542-1-AP targets FANCA-Specific in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: Fanconi anemia, complementation group A
• Calculated Molecular Weight: 163 kDa
• Observed Molecular Weight: 163 kDa
• GenBank Accession Number: NM_000135
• Gene Symbol: FANCA
• Gene ID (NCBI): 2175
• RRID: AB_10638755
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FANCA, also named as FAA, FACA and FANCH, is DNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. FANCA may be involved in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability. Defects in FANCA are a cause of Fanconi anemia (FA) which is a genetically heterogeneous, autosomal recessive disorder characterized by progressive pancytopenia, a diverse assortment of congenital malformations, and a predisposition to the development of malignancies. The antibody is specific to isoform1 of FANCA.