FAM84B Polyclonal antibody

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  WB analysis of MDA-MB-453s using 18421-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 18421-1-AP (FAM84B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 18421-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 18421-1-AP (FAM84B antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of HUVEC using 18421-1-AP

  IP result of anti-FAM84B (IP:18421-1-AP, 4ug; Detection:18421-1-AP 1:300) with HUVEC cells lysate 3600ug.

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  IHC staining of human liver cancer using 18421-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18421-1-AP (FAM84B Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver cancer using 18421-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18421-1-AP (FAM84B Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human ovary using 18421-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 18421-1-AP (FAM84B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary using 18421-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 18421-1-AP (FAM84B antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human oesophagus cancer using 18421-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer using 18421-1-AP (FAM84B antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human oesophagus cancer using 18421-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer using 18421-1-AP (FAM84B antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 18421-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 18421-1-AP (FAM84B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 18421-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 18421-1-AP (FAM84B antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 18421-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 18421-1-AP (FAM84B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 18421-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 18421-1-AP (FAM84B antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 18421-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18421-1-AP (FAM84B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 18421-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18421-1-AP (FAM84B antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 18421-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18421-1-AP (FAM84B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 18421-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18421-1-AP (FAM84B antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skin using 18421-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 18421-1-AP (FAM84B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin using 18421-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 18421-1-AP (FAM84B antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 18421-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18421-1-AP (FAM84B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 18421-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18421-1-AP (FAM84B antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 18421-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 18421-1-AP (FAM84B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 18421-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 18421-1-AP (FAM84B antibody) at dilution of 1:50 (under 40x lens).

• Featured Product
• KD/KO Validated

FAM84B Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 18421-1-AP

Publications(12)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, IP, ELISA

Synonyms

FAM84B, NSE2, Protein FAM84B, Protein NSE2

Formulation:  PBS and Azide

PBS and Azide

Conjugate:  Unconjugated

Unconjugated

Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Tested Applications

• Positive WB detected in: MDA-MB-453s cells, Jurkat cells
• Positive IP detected in: HUVEC cells
• Positive IHC detected in: human liver cancer tissue, human heart tissue, human kidney tissue, human liver tissue, human lung tissue, human oesophagus cancer tissue, human ovary tissue, human skin tissue, human spleen tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 8 publications below
• IHC: See 10 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Product Information

18421-1-AP targets FAM84B in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FAM84B fusion protein Ag13243
• Full Name: family with sequence similarity 84, member B
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC052957
• Gene Symbol: FAM84B
• Gene ID (NCBI): 157638
• RRID: AB_2278161
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96KN1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IHC	Nat Genet; Genome-wide profiling of HPV integration in cervical cancer identifies clustered genomic hot spots and a potential microhomology-mediated integration mechanism.; Authors - Zheng Hu
human	WB,IHC	Gigascience; Genomic analyses reveal FAM84B and the NOTCH pathway are associated with the progression of esophageal squamous cell carcinoma.; Authors - Caixia Cheng ; KD Validated
human	IF	Proc Natl Acad Sci U S A; An in vitro vesicle formation assay reveals cargo clients and factors that mediate vesicular trafficking.; Authors - Yan Huang ; KD Validated
human	WB,IHC	Ther Adv Med Oncol; FAM84B promotes prostate tumorigenesis through a network alteration.; Authors - Yanzhi Jiang
human	IHC	Aging (Albany NY); FAM84B, amplified in pancreatic ductal adenocarcinoma, promotes tumorigenesis through the Wnt/β-catenin pathway.; Authors - Xin Zhang
human,mouse	WB,IHC	Oncotarget; Upregulation of FAM84B during prostate cancer progression.; Authors - Nicholas Wong