FAM83G Polyclonal antibody

FAM83G Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 29366-1-AP

Synonyms

Protein FAM83G

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Validation Data Gallery

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  WB analysis using 29366-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29366-1-AP (FAM83G antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: BxPC-3 cells, HCT 116 cells, HepG2 cells, U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29366-1-AP targets FAM83G in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FAM83G fusion protein Ag28925
• Full Name: family with sequence similarity 83, member G
• Calculated Molecular Weight: 91 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: NM_001039999
• Gene Symbol: FAM83G
• Gene ID (NCBI): 644815
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FAM83G is a member of the FAM83 protein family, which consists of eight members characterized by sharing a conserved N-terminal domain named DUF1669. This domain is homologous to the phospholipase D catalytic domain but lacks essential catalytic histidine residues, suggesting it is unlikely to have phospholipase activity. FAM83G, in particular, has a consensus motif for serine/threonine protein kinase D1/protein kinase C mu (PKD1/PKCμ) phosphorylation at serine residue 356 (S356), which suggests it may have a unique physiological function among the FAM83 protein family members. Furthermore, FAM83G has been identified as a novel inducer of apoptosis, with its phosphorylation at S356 modulating heat shock protein 27 (HSP27) phosphorylation and apoptosis regulation.