FAM3C Monoclonal antibody, PBS Only

FAM3C Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2E10D5

Cat no : 60282-1-PBS

Synonyms

FAM3C, GS3786, ILEI, Interleukin like EMT inducer, Protein FAM3C

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Validation Data Gallery

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  WB analysis of fetal human brain using 60282-1-Ig (same clone as 60282-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60282-1-Ig (FAM3C Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60282-1-PBS in a different storage buffer formulation.

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  IHC staining of human oesophagus cancer using 60282-1-Ig (same clone as 60282-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60282-1-Ig (FAM3C Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60282-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human oesophagus cancer using 60282-1-Ig (same clone as 60282-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60282-1-Ig (FAM3C Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60282-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 60282-1-Ig (same clone as 60282-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed HepG2 cells using 60282-1-Ig(FAM3C antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60282-1-PBS in a different storage buffer formulation.

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Product Information

60282-1-PBS targets FAM3C in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: FAM3C fusion protein Ag5516
• Full Name: family with sequence similarity 3, member C
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC046932
• Gene Symbol: FAM3C
• Gene ID (NCBI): 10447
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

FAM3C, also known as ILEI, is a secreted protein belonging to the cytokine-like protein family. FAM3C is a signaling cytokine with a GG domain, hydrophobic leader sequence and an N-terminal signal peptide. A change in expression of this protein has been noted in pancreatic cancer-derived cells. FAM3C has been involved in the epithelial to mesenchymal transition, and retinal laminar formation processes in vertebrates. Also, FAM3C may play an important role in inner ear development and thus be involved in cell differentiation and proliferation during inner ear embryogenesis.