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FAM134B Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human

Detection: human

Applications

Cytometric bead array, Sandwich ELISA

Range

Cytometric Bead Array: 0.781-100 ng/mL

Sandwich ELISA: 0.625-40 ng/mL

Cat No : MP01561-1

Print datasheet

Synonyms

Validation Data Gallery

  • Sandwich ELISA standard curve of MP01561-1, Human FAM134B Recombinant Matched Antibody Pair - PBS only. 84773-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag16178. 84773-2-PBS was HRP conjugated as the detection antibody. Range: 0.625-40 ng/mL.

    Sandwich ELISA standard curve of MP01561-1

    Sandwich ELISA standard curve of MP01561-1, Human FAM134B Recombinant Matched Antibody Pair - PBS only. 84773-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag16178. 84773-2-PBS was HRP conjugated as the detection antibody. Range: 0.625-40 ng/mL.

  • The mean FAM134B concentration was determined to be 106.63 ng/mL in HeLa cell extract based on a 1.00 mg/mL extract load and 354.78 ng/mL in HCT 116 cell extract based on a 2.00 mg/mL extract load.

    Sample test of MP01561-1

    The mean FAM134B concentration was determined to be 106.63 ng/mL in HeLa cell extract based on a 1.00 mg/mL extract load and 354.78 ng/mL in HCT 116 cell extract based on a 2.00 mg/mL extract load.

  • Cytometric bead array standard curve of MP01561-1, FAM134B Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84773-1-PBS. Detection antibody: 84773-2-PBS. Standard: Ag16178. Range: 0.781-100 ng/mL.

    Cytometric bead array standard curve of MP01561-1

    Cytometric bead array standard curve of MP01561-1, FAM134B Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84773-1-PBS. Detection antibody: 84773-2-PBS. Standard: Ag16178. Range: 0.781-100 ng/mL.

Tested Applications

Cytometric bead array, Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP01561-1 targets FAM134B in immunoassays as a matched antibody pair. Validated in Cytometric bead array, Sandwich ELISA.

Range: 0.781-100 ng/mL (Cytometric Bead Array); 0.625-40 ng/mL (Sandwich ELISA);

Capture antibody: FAM134B Recombinant antibody, PBS Only (Capture) 84773-1-PBS (242216A5). 100 μg. Concentration 1 mgl/ml.

Detection antibody: FAM134B Recombinant antibody, PBS Only (Detector) 84773-2-PBS (242216D6). 100 μg. Concentration 1 mgl/ml.

Alternative FAM134B matched antibody pairs: MP50748-1, MP50748-2

Matched pair protein standard: Ag16178

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 84773-1-PBS
Tested Reactivity human
Immunogen FAM134B fusion protein Ag16178
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name family with sequence similarity 134, member B
Synonyms RETREG1, Reticulophagy regulator 1, Reticulophagy receptor 1, JK1, 242216A5
GenBank accession numberBC030517
Gene symbol FAM134B
UniprotQ9H6L5
Gene ID (NCBI) 54463
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 84773-2-PBS
Tested Reactivity human
Immunogen FAM134B fusion protein Ag16178
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name family with sequence similarity 134, member B
Synonyms RETREG1, Reticulophagy regulator 1, Reticulophagy receptor 1, JK1, 242216D6
GenBank accession numberBC030517
Gene symbol FAM134B
UniprotQ9H6L5
Gene ID (NCBI) 54463
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.