FAM129A Polyclonal antibody

• Featured Product
• KD/KO Validated

FAM129A Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 21333-1-AP

Synonyms

Protein FAM129A, NIBAN1, NIBAN, GIG39, Cell growth-inhibiting gene 39 protein

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Validation Data Gallery

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  WB analysis using 21333-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21333-1-AP (FAM129A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 21333-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FAM129A antibody (21333-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: A431 cells, DU 145 cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 4 publications below
• IF: See 2 publications below

Product Information

21333-1-AP targets FAM129A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FAM129A fusion protein Ag15413
• Full Name: family with sequence similarity 129, member A
• Calculated Molecular Weight: 928 aa, 103 kDa
• Observed Molecular Weight: 150 kDa
• GenBank Accession Number: BC030531
• Gene Symbol: FAM129A
• Gene ID (NCBI): 116496
• RRID: AB_10733106
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB, IF	Front Cell Dev Biol; The Regulatory Role of GBF1 on Osteoclast Activation Through EIF2a Mediated ER Stress and Novel Marker FAM129A Induction.; Authors - Cailing Wen
human	WB,IHC	J Cancer; Niban apoptosis regulator 1 promotes gemcitabine resistance by activating the focal adhesion kinase signaling pathway in bladder cancer.; Authors - Shiyu Tong ; KD Validated
mouse	WB,IHC	Ren Fail; Niban protein regulates apoptosis in HK-2 cells via caspase-dependent pathway.; Authors - Shiqi Tang
human	WB	Cell Death Discov; miR-4521-FAM129A axial regulation on ccRCC progression through TIMP-1/MMP2/MMP9 and MDM2/p53/Bcl2/Bax pathways.; Authors - Xue Feng
human	WB,IHC,IF	J Adv Res; MiR-4521 plays a tumor repressive role in growth and metastasis of hepatocarcinoma cells by suppressing phosphorylation of FAK/AKT pathway via targeting FAM129A.; Authors - Munawar Ayesha ; KD Validated
human,mouse	WB,IHC	Int J Oral Sci; Phenformin activates ER stress to promote autophagic cell death via NIBAN1 and DDIT4 in oral squamous cell carcinoma independent of AMPK; Authors - Dexuan Zhuang