FAM127B Recombinant antibody

FAM127B Uni-rAb^TM Recombinant Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Conjugate

Unconjugated

CloneNo.

240716D8

Cat no : 83862-1-RR

Synonyms

FAM127 A/B/C, FAM127, FAM127C, FAM127A, CXX1

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Validation Data Gallery

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  WB analysis using 83862-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83862-1-RR (FAM127B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 83862-1-RR

  IP result of anti-FAM127B (IP:83862-1-RR, 4ug; Detection:83862-1-RR 1:800) with HeLa cells lysate 1280 ug.

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  Affinity and Kinetic Characterization of 83862-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83862-1-RR against Human FAM127B were performed. The affinity constant is 13.3 nM.

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, A549 cells, MCF-7 cells, A2780 cells, U-251 cells
• Positive IP detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83862-1-RR is FAM127B Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: FAM127B fusion protein Ag13930
• Full Name: family with sequence similarity 127, member B
• Calculated Molecular Weight: 113 aa, 13 kDa
• Observed Molecular Weight: 13 kDa
• GenBank Accession Number: BC000393
• Gene Symbol: FAM127B
• Gene ID (NCBI): 26071
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The FAM127 family currently has three members, namely FAM127A (also called RTL8C and CXX1), FAM127B (also called CXX1B and RTL8A), and FAM127C (also called RTL8B and CXX1C). They are located on chromosomes and their functions are unknown. All three are composed of 113 amino acids and have extremely high sequence similarity. The immunogen of this antibody has a sequence similarity of more than 95% with members of the FAM127 family. Theoretically, the antibody can recognize all members of the FAM127 family.