PC-3 cells were subjected to SDS PAGE followed by western blot with 83473-3-RR (FAM116A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
PC-3 cells were subjected to SDS PAGE followed by western blot with 83473-3-RR (FAM116A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 83473-3-RR
HEK-293 cells were subjected to SDS PAGE followed by western blot with 83473-3-RR (FAM116A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
HEK-293 cells were subjected to SDS PAGE followed by western blot with 83473-3-RR (FAM116A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
FC experiment of HepG2 using 83473-3-RR
1x10^6 HepG2 cells were intracellularly stained with 0.25 ug FAM116A Recombinant antibody (83473-3-RR, Clone:240486B9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
1x10^6 HepG2 cells were intracellularly stained with 0.25 ug FAM116A Recombinant antibody (83473-3-RR, Clone:240486B9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
Affinity and Kinetic Characterization of 83473-3-RR
Biolayer interferometry (BLl) kinetic assays of 83473-3-RR against Human FAM116A were performed. The affinity constant is 1.43 nM.
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
PC-3 cells were subjected to SDS PAGE followed by western blot with 83473-3-RR (FAM116A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 83473-3-RR
HEK-293 cells were subjected to SDS PAGE followed by western blot with 83473-3-RR (FAM116A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
FC (INTRA) Figures
FC experiment of HepG2 using 83473-3-RR
1x10^6 HepG2 cells were intracellularly stained with 0.25 ug FAM116A Recombinant antibody (83473-3-RR, Clone:240486B9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
AFFINITY Figures
Affinity and Kinetic Characterization of 83473-3-RR
Biolayer interferometry (BLl) kinetic assays of 83473-3-RR against Human FAM116A were performed. The affinity constant is 1.43 nM.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
FAM116A Recombinant antibody
Catalog Number
83473-3-RR
Citations
-
Dilutions
WB : 1:2000-1:10000 FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
Applications
WB, FC (Intra), ELISA
Reactivity
human
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
