CoraLite®555-conjugated FAK Monoclonal antibody

• Featured Product
• KD/KO Validated

FAK Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2c

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

2C5B9

Cat no : CL555-66258

Synonyms

FADK, FADK 1, FAK, FAK1, Focal adhesion kinase 1, pp125FAK, Protein tyrosine kinase 2, PTK2, PTK2 protein tyrosine kinase 2

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Validation Data Gallery

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  IF Staining of A549 using CL555-66258

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CoraLite®555 FAK antibody (CL555-66258, Clone: 2C5B9 ) at dilution of 1:200.

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  IF Staining of HUVEC using CL555-66258

  Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using CoraLite®555 FAK antibody (CL555-66258, Clone: 2C5B9 ) at dilution of 1:200, CL488-Phalloidin (green).

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  IF Staining of A549 using CL555-66258

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CoraLite®555 FAK antibody (CL555-66258, Clone: 2C5B9 ) at dilution of 1:200, CoraLite®488 Beta Actin antibody (CL488-66009, Clone: 2D4H5, green).

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Tested Applications

• Positive IF/ICC detected in: A549 cells, HUVEC cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL555-66258 targets FAK in IF/ICC applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2c
• Class: Monoclonal
• Type: Antibody
• Immunogen: FAK fusion protein Ag17966
• Full Name: PTK2 protein tyrosine kinase 2
• Calculated Molecular Weight: 1052 aa, 119 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC028733
• Gene Symbol: FAK
• Gene ID (NCBI): 5747
• RRID: AB_2919682
• Conjugate: CoraLite®555 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 557 nm / 570nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage.

Background Information

FAK (Focal adhesion kinase 1) is also named as FAK1, FADK, pp125FAK, FAK and belongs to the protein kinase superfamily. It is a critical tyrosine kinase that modulates cell adhesion, migration, proliferation and survival in response to extracellular signals (PMID:19664602). It also acts as a pivotal signal 'integrator', controlling and coordinating cellular responses that include cell migration, survival, proliferation and, epithelial tissue repair after DNA damage (PMID:20966971). This protein has some isoforms produced by alternative promoter usage and alternative splicing, and the range of the molecular weights are 100-120kD and 40-60kD.