Phospho-FAK (Tyr397) Recombinant antibody, PBS Only

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  WB analysis using 83933-1-RR (same clone as 83933-1-PBS)

  Non-treated and pervanadate treated HeLa cells were subjected to SDS PAGE followed by western blot with 83933-1-RR (Phospho-FAK (Tyr397) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 83933-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 83933-1-RR (same clone as 83933-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using Phospho-FAK (Tyr397) antibody (83933-1-RR, Clone: 240630F8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83933-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A549 using 83933-1-RR (same clone as 83933-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using Phospho-FAK (Tyr397) antibody (83933-1-RR, Clone: 240630F8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83933-1-PBS in a different storage buffer formulation.

Phospho-FAK (Tyr397) {ptg:Brand} Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 83933-1-PBS

Clone No.240630F8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

FAK (Tyr397), p FAK, p FAK (Tyr397), p FAK Tyr397, p-FAK

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Product Information

83933-1-PBS targets Phospho-FAK (Tyr397) in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: PTK2 protein tyrosine kinase 2
• Calculated Molecular Weight: 1052 aa, 119 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC028733
• Gene Symbol: FAK
• Gene ID (NCBI): 5747
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q05397
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

FAK (Focal adhesion kinase 1) is also named as FAK1, FADK, pp125FAK, FAK and belongs to the protein kinase superfamily. It is a critical tyrosine kinase that modulates cell adhesion, migration, proliferation and survival in response to extracellular signals (PMID:19664602). It also acts as a pivotal signal 'integrator', controlling and coordinating cellular responses that include cell migration, survival, proliferation and, epithelial tissue repair after DNA damage (PMID:20966971). This protein has some isoforms produced by alternative promoter usage and alternative splicing, and the range of the molecular weights are 100-120 kDa and 40-60 kDa.