FAK Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

FAK Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2c

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2C5B9

Cat no : 66258-1-PBS

Synonyms

FADK, FADK 1, FAK, FAK1, Focal adhesion kinase 1, pp125FAK, Protein tyrosine kinase 2, PTK2, PTK2 protein tyrosine kinase 2

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Validation Data Gallery

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  WB analysis using 66258-1-Ig (same clone as 66258-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66258-1-Ig (FAK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 66258-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 66258-1-Ig (same clone as 66258-1-PBS)

  WB result of FAK antibody (66258-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FAK transfected MCF-7 cells. This data was developed using the same antibody clone with 66258-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 66258-1-Ig (same clone as 66258-1-PBS)

  Western blot analysis of FAK-specific in various cell lines using Proteintech antibody 66258-1-Ig at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66258-1-PBS in a different storage buffer formulation.

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  WB analysis using 66258-1-Ig (same clone as 66258-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66258-1-Ig (FAK antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66258-1-PBS in a different storage buffer formulation.

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  WB analysis of human testis using 66258-1-Ig (same clone as 66258-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 66258-1-Ig (FAK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66258-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66258-1-Ig (same clone as 66258-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66258-1-Ig (FAK antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66258-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66258-1-Ig (same clone as 66258-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66258-1-Ig (FAK antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66258-1-PBS in a different storage buffer formulation.

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  IF Staining of HUVEC using 66258-1-Ig (same clone as 66258-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using FAK antibody (66258-1-Ig, Clone: 2C5B9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66258-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 66258-1-Ig (same clone as 66258-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using FAK antibody (66258-1-Ig, Clone: 2C5B9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5, red). This data was developed using the same antibody clone with 66258-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66258-1-PBS targets FAK in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2c
• Class: Monoclonal
• Type: Antibody
• Immunogen: FAK fusion protein Ag17966
• Full Name: PTK2 protein tyrosine kinase 2
• Calculated Molecular Weight: 1052 aa, 119 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC028733
• Gene Symbol: PTK2
• Gene ID (NCBI): 5747
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

FAK (Focal adhesion kinase 1) is also named as FAK1, FADK, pp125FAK, FAK and belongs to the protein kinase superfamily. It is a critical tyrosine kinase that modulates cell adhesion, migration, proliferation and survival in response to extracellular signals (PMID:19664602). It also acts as a pivotal signal 'integrator', controlling and coordinating cellular responses that include cell migration, survival, proliferation and, epithelial tissue repair after DNA damage (PMID:20966971). This protein has some isoforms produced by alternative promoter usage and alternative splicing, and the range of the molecular weights are 100-120kD and 40-60kD.