FADS3 Polyclonal antibody

FADS3 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 15205-1-AP

Synonyms

fatty acid desaturase 3, FADS 3, EC:1.14.19.-, Delta(13) fatty acid desaturase (Delta(13) desaturase ), Cytochrome b5 related protein

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Validation Data Gallery

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  WB analysis using 15205-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15205-1-AP (FADS3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 15205-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15205-1-AP (FADS3 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15205-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15205-1-AP (FADS3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 15205-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15205-1-AP (FADS3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of L02 using 15205-1-AP

  IP result of anti-FADS3 (IP:15205-1-AP, 4ug; Detection:15205-1-AP 1:500) with L02 cells lysate 3000 ug.

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  IHC staining of human ovary cancer using 15205-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 15205-1-AP (FADS3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse skeletal muscle tissue, mouse kidney tissue, L02 cells, rat skeletal muscle tissue, mouse liver tissue
• Positive IP detected in: L02 cells
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15205-1-AP targets FADS3 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FADS3 fusion protein Ag7355
• Full Name: fatty acid desaturase 3
• Calculated Molecular Weight: 51 kDa
• Observed Molecular Weight: 41-51 kDa
• GenBank Accession Number: BC004901
• Gene Symbol: FADS3
• Gene ID (NCBI): 3995
• RRID: AB_10859776
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FADS3, also named as CYB5RP, belongs to fatty acid desaturase (FADS) gene family. Desaturase enzymes regulate unsaturation of fatty acids through the introduction of double bonds between defined carbons of the fatty acyl chain. FADS family memebers are considered fusion products composed of an N-terminal cytochrome b5-like domain and a C-terminal multiple membrane-spanning desaturase protion, both of which are characterized by conserved histidine motifs. FADS3 can be detected as 40 kDa-51 kDa.