FADS1 Polyclonal antibody

• Featured Product
• KD/KO Validated

FADS1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

12

Cat no : 10627-1-AP

Synonyms

FADSD5, EC:1.14.19.44, Delta-5 desaturase, D5D, Acyl-CoA (8-3)-desaturase

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Validation Data Gallery

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  WB analysis using 10627-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10627-1-AP (FADS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10627-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10627-1-AP (FADS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 10627-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10627-1-AP (FADS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 10627-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10627-1-AP (FADS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human endometrial cancer using 10627-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 10627-1-AP (FADS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 10627-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10627-1-AP (FADS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 10627-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10627-1-AP (FADS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human endometrial cancer using 10627-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 10627-1-AP (FADS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of SH-SY5Y using 10627-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed SH-SY5Y cells using 10627-1-AP (FADS1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: L02 cells, HeLa cells, Jurkat cells, mouse liver tissue
• Positive IHC detected in: human colon cancer tissue, human lung cancer tissue, human endometrial cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

10627-1-AP targets FADS1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FADS1 fusion protein Ag0978
• Full Name: fatty acid desaturase 1
• Calculated Molecular Weight: 52 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC007846
• Gene Symbol: FADS1
• Gene ID (NCBI): 3992
• RRID: AB_2231403
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FADS1, also known as Delta-5 desaturase or D5D, is a 444 amino acid protein that belongs to the fatty acid desaturase (FADS) gene family. Desaturase enzymes regulate unsaturation of fatty acids through the introduction of double bonds between defined carbons of the fatty acyl chain. FADS1 is a key enzyme in polyunsaturated fatty acid (PUFA) metabolism that catalyze the conversion of linoleic acid (LA) into arachidonic acid (AA) and that of alpha-linolenic acid (ALA) into eicosapentaenoic acid (EPA).

Publications

Species	Application	Title
human	WB	Cell Metab; STING orchestrates the crosstalk between polyunsaturated fatty acid metabolism and inflammatory responses.; Authors - Isabelle K Vila
human	WB	Nat Commun; The lipoprotein-associated phospholipase A2 inhibitor Darapladib sensitises cancer cells to ferroptosis by remodelling lipid metabolism; Authors - Mihee Oh
human	WB	Proc Natl Acad Sci U S A; Polyunsaturated fatty acid biosynthesis pathway determines ferroptosis sensitivity in gastric cancer.; Authors - Ji-Yoon Lee
human	IHC	Stem Cells; Increased lipogenesis is critical for self-renewal and growth of breast cancer stem cells: Impact of omega-3 fatty acids.; Authors - Haiqing Luo
mouse	WB	J Lipid Res; In vivo MRS assessment of altered fatty acyl unsaturation in liver tumor formation of a TGF alpha/c-myc transgenic mouse model.; Authors - Griffitts J J
human	IHC	J Cancer; Reduced Expression of FADS1 Predicts Worse Prognosis in Non-Small-Cell Lung Cancer.; Authors - Dong Wang