FABP6 Polyclonal antibody

• Featured Product
• KD/KO Validated

FABP6 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA, IF

Conjugate

Unconjugated

Publications

6

Cat no : 13781-1-AP

Synonyms

FABP6, Fatty acid binding protein 6, Gastrotropin, GT, I 15P, I BABP, I BALB, I BAP, ILBP, ILBP3, Ileal lipid binding protein, ILLBP, Intestinal 15 kDa protein

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Validation Data Gallery

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  WB analysis of mouse small intestine using 13781-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 13781-1-AP (FABP6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human small intestine using 13781-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 13781-1-AP (FABP6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human small intestine using 13781-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 13781-1-AP (FABP6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse small intestine using 13781-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 13781-1-AP (FABP6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse small intestine using 13781-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 13781-1-AP (FABP6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse small intestine tissue
• Positive IHC detected in: human small intestine tissue, mouse small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Product Information

13781-1-AP targets FABP6 in WB, IHC, ELISA, IF applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FABP6 fusion protein Ag4788
• Full Name: fatty acid binding protein 6, ileal
• Calculated Molecular Weight: 177 aa, 20 kDa
• Observed Molecular Weight: 14 kDa
• GenBank Accession Number: BC022489
• Gene Symbol: FABP6
• Gene ID (NCBI): 2172
• RRID: AB_2877976
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fatty acid binding protein 6 (FABP6, also known as the ileal bile acid binding protein IBABP) is regarded as a bile acid binding protein found in the distal portion of the small intestine and may be important in maintaining bile acid homeostasis(PMID: 25754072). FABP6 is reportedly up-regulated in colorectal cancer, it has been suggested as a link between bile acids and the risk of colorectal cancer(PMID: 17909007). And also, it was showed a potential drug target for the treatment of diabetes(PMID: 27500412). There are 2 isoforms of this protein,one of which is about 14 kDa we detected.

Publications

Species	Application	Title
human	IF	J Exp Med; Single-cell transcriptome analysis reveals differential nutrient absorption functions in human intestine.; Authors - Yalong Wang
mouse	WB	Biomaterials; MMP-12 siRNA improves the homeostasis of the small intestine and metabolic dysfunction in high-fat diet feeding-induced obese mice.; Authors - Mingming Song
mouse	WB	J Agric Food Chem; Novel Metabolic Regulation of Bile Acid Responses to Low Cholesterol in Whole-Grain-Diet-Fed Mice.; Authors - Jinxin Liu
human	WB,IHC	J Immunol Res; FABP6 Expression Correlates with Immune Infiltration and Immunogenicity in Colorectal Cancer Cells; Authors - Wenping Lian ; KD Validated
human	WB,IHC,IF	Aging (Albany NY); FABP6 serves as a new therapeutic target in esophageal tumor; Authors - Dengfeng Zhang
human	WB	Int Immunopharmacol; Melatonin alleviates necrotizing enterocolitis by reducing bile acid levels through the SIRT1/FXR signalling axis; Authors - Jiahao Lai