FABP5 Monoclonal antibody

• Featured Product
• KD/KO Validated

FABP5 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

3

CloneNo.

1C6E12

Cat no : 66299-1-Ig

Synonyms

Fatty acid-binding protein, epidermal, Fatty acid-binding protein 5, Fatty acid binding protein 5, Epidermal-type fatty acid-binding protein, EFABP

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Validation Data Gallery

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  WB analysis of A375 using 66299-1-Ig

  WB result of FABP5 antibody (66299-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FABP5 transfected A375 cells.

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  WB analysis using 66299-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66299-1-Ig (FABP5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis of A375 using 66299-1-Ig

  A375 cells were subjected to SDS PAGE followed by western blot with 66299-1-Ig (FABP5 Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis using 66299-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66299-1-Ig (FABP5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of fetal human brain using 66299-1-Ig

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66299-1-Ig (FABP5 Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brown adipose using 66299-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 66299-1-Ig (FABP5 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brown adipose using 66299-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 66299-1-Ig (FABP5 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 66299-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66299-1-Ig (FABP5 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 66299-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66299-1-Ig (FABP5 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 66299-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66299-1-Ig (FABP5 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 66299-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66299-1-Ig (FABP5 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 66299-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using FABP5 antibody (66299-1-Ig, Clone: 1C6E12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: A375 cells, U2OS cells, rat brain tissue, fetal human brain tissue, A549 cells, HeLa cells, HEK-293 cells, mouse brain tissue
• Positive IHC detected in: human breast cancer tissue, human prostate cancer tissue, mouse brown adipose tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochemistry (IHC): IHC : 1:200-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 2 publications below

Product Information

66299-1-Ig targets FABP5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: FABP5 fusion protein Ag3005
• Full Name: fatty acid binding protein 5 (psoriasis-associated)
• Calculated Molecular Weight: 135 aa, 15 kDa
• Observed Molecular Weight: 15 kDa
• GenBank Accession Number: BC019385
• Gene Symbol: FABP5
• Gene ID (NCBI): 2171
• RRID: AB_2881682
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FABP5, also named as PA-FABP and E-FABP, belongs to the calycin superfamily and Fatty-acid binding protein (FABP) family. It is high specificity for fatty acids. FABP5 is highest affinity for C18 chain length. It may be involved in keratinocyte differentiation. FABP5 is a fatty acid-binding protein and is expressed in epidermis and endothelial cells of the microvasculature of different organs. FABP5 has also been identified as a tumor-associated antigen, which is highly expressed in various cancers. FABP5 was detected in the sera of HNSCC patients with early stage cancer. Antibodies specific for FABP5 were significantly increased in a substantial amount in patients, suggesting that FABP5 may be a potential diagnostic biomarker for HNSCC. FABP5 may serve as a biomarker for HNSCC.(PMID:19602232)

Publications

Species	Application	Title
human	WB	Front Oncol; Multi-Omics Analysis of Fatty Acid Metabolism in Thyroid Carcinoma.; Authors - Jinghui Lu
human	WB,IHC	Carcinogenesis; Serum fatty acid-binding protein 5 is a significant factor in hepatocellular carcinoma progression independent of tissue expression level.; Authors - Masafumi Ohira ; KO Validated
human	IHC	Med Mol Morphol; Expression of fatty-acid-binding protein 5 in intrahepatic and extrahepatic cholangiocarcinoma: the possibility of different energy metabolisms in anatomical location.; Authors - Risa Nakagawa