FABP2 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

FABP2 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, rabbit, pig

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2D11G6

Cat no : 67691-1-PBS

Synonyms

FABP2, FABPI, Fatty acid binding protein 2, I FABP

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Validation Data Gallery

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  WB analysis using 67691-1-Ig (same clone as 67691-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67691-1-Ig (FABP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67691-1-PBS in a different storage buffer formulation.

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  WB analysis of COLO 320 using 67691-1-Ig (same clone as 67691-1-PBS)

  WB result of FABP2 antibody (67691-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FABP2 transfected COLO 320 cells. This data was developed using the same antibody clone with 67691-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67691-1-Ig (same clone as 67691-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67691-1-Ig (FABP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67691-1-PBS in a different storage buffer formulation.

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  IHC staining of human small intestine using 67691-1-Ig (same clone as 67691-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 67691-1-Ig (FABP2 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67691-1-PBS in a different storage buffer formulation.

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  IHC staining of human small intestine using 67691-1-Ig (same clone as 67691-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 67691-1-Ig (FABP2 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67691-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse small intestine using 67691-1-Ig (same clone as 67691-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 67691-1-Ig (FABP2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67691-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse small intestine using 67691-1-Ig (same clone as 67691-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 67691-1-Ig (FABP2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67691-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat small intestine using 67691-1-Ig (same clone as 67691-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 67691-1-Ig (FABP2 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67691-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse colon using 67691-1-Ig (same clone as 67691-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 67691-1-Ig (FABP2 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67691-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse colon using 67691-1-Ig (same clone as 67691-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using FABP2 antibody (67691-1-Ig, Clone: 2D11G6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67691-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67691-1-PBS targets FABP2 in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat, rabbit, pig samples.

• Tested Reactivity: Human, mouse, rat, rabbit, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: FABP2 fusion protein Ag17620
• Full Name: fatty acid binding protein 2, intestinal
• Calculated Molecular Weight: 132 aa, 15 kDa
• Observed Molecular Weight: 15 kDa
• GenBank Accession Number: BC069617
• Gene Symbol: FABP2
• Gene ID (NCBI): 2169
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

Background Information

FABP2, also known as the intestinal fatty acid binding protein (I-FABP), is expressed in the absorptive intestinal villus cells. It is mainly involved in intracellular transport and intestinal absorption of lipids. FABP2 has been considered a marker of mucosal injury and ischemia and serum I-FABP level is used as a tissue damage indicator. In addition, it is a marker of differentiated intestinal epithelial cells.