F7 Polyclonal antibody

F7 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 23058-1-AP

Synonyms

Factor VII, SPCA, Proconvertin, Eptacog alfa, EC:3.4.21.21

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Validation Data Gallery

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  WB analysis using 23058-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23058-1-AP (F7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 23058-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 23058-1-AP (F7 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 23058-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 23058-1-AP (F7 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human plasma using 23058-1-AP

  human plasma were subjected to SDS PAGE followed by western blot with 23058-1-AP (F7 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF Staining of HUVEC using 23058-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using F7 antibody (23058-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

• Positive WB detected in: mouse liver tissue, human placenta tissue, human plasma
• Positive IF/ICC detected in: HUVEC cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

23058-1-AP targets F7 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: F7 fusion protein Ag19324
• Full Name: coagulation factor VII (serum prothrombin conversion accelerator)
• Calculated Molecular Weight: 466 aa, 52 kDa
• Observed Molecular Weight: 49-55 kDa
• GenBank Accession Number: BC130468
• Gene Symbol: F7
• Gene ID (NCBI): 2155
• RRID: AB_2879203
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

F7(Coagulation factor VII) is also named as serum prothrombin conversion accelerator(SPCA). It is the key triggering enzyme of the extrinsic coagulation pathway. Hepatocytes are the predominant source of F7 production under normal physiologic conditions, although F7 is also expressed by monocytes/macrophages in inflammation and atherosclerosis(PMID:17018600). This protein has 2 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	WB	Sci Rep; C-reactive protein (CRP) recognizes uric acid crystals and recruits proteases C1 and MASP1.; Authors - Anika Alberts
mouse	WB	Drug Des Devel Ther; Guanxinning Tablet Alleviates Post-Ischemic Stroke Injury Via Regulating Complement and Coagulation Cascades Pathway and Inflammatory Network Mobilization; Authors - Yule Wang