Mouse F4/80 Recombinant antibody, PBS Only

F4/80 Uni-rAb^TM Recombinant Antibody for IHC, IF/ICC, IF-P, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

IHC, IF/ICC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2P18

Cat no : 81668-1-PBS

Synonyms

Emr1, EGF-like module-containing mucin-like hormone receptor-like 1, EGF-like module receptor 1, Adhesion G protein-coupled receptor E1, Adgre1

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Validation Data Gallery

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  IHC staining of mouse spleen using 81668-1-RR (same clone as 81668-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 81668-1-RR (F4/80 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81668-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 81668-1-RR (same clone as 81668-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 81668-1-RR (F4/80 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81668-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 81668-1-RR (same clone as 81668-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 81668-1-RR (F4/80 antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81668-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 81668-1-RR (same clone as 81668-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 81668-1-RR (F4/80 antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81668-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse spleen using 81668-1-RR (same clone as 81668-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using F4/80 antibody (81668-1-RR, Clone: 2P18 ) at dilution of 1:1500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81668-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse spleen using 81668-1-RR (same clone as 81668-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using F4/80 antibody (81668-1-RR, Clone: 2P18 ) at dilution of 1:1500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81668-1-PBS in a different storage buffer formulation.

• 

  IF Staining of RAW 264.7 using 81668-1-RR (same clone as 81668-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed RAW 264.7 cells using F4/80 antibody (81668-1-RR, Clone: 2P18 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 81668-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 81668-1-RR

  Biolayer interferometry (BLl) kinetic assays of 81668-1-RR against Mouse F4/80 were performed. The affinity constant is 14.4 nM.

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Product Information

81668-1-PBS targets F4/80 in IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: F4/80 fusion protein Ag28252
• Full Name: EGF-like module containing, mucin-like, hormone receptor-like sequence 1
• Calculated Molecular Weight: 102 kDa
• GenBank Accession Number: NM_010130
• Gene Symbol: F4/80
• Gene ID (NCBI): 13733
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Mouse F4/80, also named as EMR1 and Gpf480, is a 160kd cell surface glycoprotein which is a member of the EGF TM7 family. The F4/80 molecule is solely expressed on the surface of macrophages and serves as a marker for mature macrophage tissues, including Kupffer cells in liver, splenic red pulp macrophages, brain microglia, gut lamina propria and Langerhans cells in the skin. The function of F4/80 is unclear, but it is speculated to be involved in macrophage adhesion events, cell migration or as a G protein-coupled signaling component of macrophages.