F4/80 Polyclonal antibody
F4/80 Polyclonal Antibody for IHC, IF-P, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
mouse, rat and More (1)
Applications
WB, IHC, IF-P, ELISA
Conjugate
Unconjugated
Cat no : 29414-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive IHC detected in | mouse spleen tissue, mouse liver tissue, rat spleen tissue, rat liver tissue, mouse lung tissue, mouse heart tissue, mouse brain tissue, mouse small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | mouse spleen tissue, mouse liver tissue |
Recommended dilution
Application | Dilution |
---|---|
Immunohistochemistry (IHC) | IHC : 1:8000-1:20000 |
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 6 publications below |
IHC | See 33 publications below |
IF | See 34 publications below |
Product Information
29414-1-AP targets F4/80 in WB, IHC, IF-P, ELISA applications and shows reactivity with mouse, rat samples.
Tested Reactivity | mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | F4/80 fusion protein Ag30149 |
Full Name | EGF-like module containing, mucin-like, hormone receptor-like sequence 1 |
Calculated Molecular Weight | 102 kDa |
GenBank Accession Number | NM_010130 |
Gene Symbol | F4/80 |
Gene ID (NCBI) | 13733 |
RRID | AB_2918300 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Mouse F4/80, also named as EMR1 and Gpf480, is a 160kd cell surface glycoprotein which is a member of the EGF TM7 family. The F4/80 molecule is solely expressed on the surface of macrophages and serves as a marker for mature macrophage tissues, including Kupffer cells in liver, splenic red pulp macrophages, brain microglia, gut lamina propria and Langerhans cells in the skin. The function of F4/80 is unclear, but it is speculated to be involved in macrophage adhesion events, cell migration or as a G protein-coupled signaling component of macrophages.
Protocols
Product Specific Protocols | |
---|---|
IHC protocol for F4/80 antibody 29414-1-AP | Download protocol |
IF protocol for F4/80 antibody 29414-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cancer Commun (Lond) Glutamine metabolic microenvironment drives M2 macrophage polarization to mediate trastuzumab resistance in HER2-positive gastric cancer | ||
J Exp Clin Cancer Res FAM171B stabilizes vimentin and enhances CCL2-mediated TAM infiltration to promote bladder cancer progression | ||
Mater Today Bio Mussel-inspired collagen-hyaluronic acid composite scaffold with excellent antioxidant properties and sustained release of a growth factor for enhancing diabetic wound healing. | ||
ACS Appl Mater Interfaces Catalysis-Mediated Male Contraception through Black Phosphorus Nanosheets | ||
Mater Today Bio Intracellular hydrogelation of macrophage conjugated probiotics for hitchhiking delivery and combined treatment of colitis | ||
EMBO Rep Eicosapentaenoic acid induces macrophage Mox polarization to prevent diabetic cardiomyopathy |