F4/80 Polyclonal antibody

F4/80 Polyclonal Antibody for IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat and More (1)

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Publications

57

Cat no : 29414-1-AP

Synonyms

F4 80, Emr1, EGF-like module-containing mucin-like hormone receptor-like 1, EGF-like module receptor 1, Adhesion G protein-coupled receptor E1

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Validation Data Gallery

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  IHC staining of mouse spleen using 29414-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 29414-1-AP (F4/80 antibody) at dilution of 1:12000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 29414-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 29414-1-AP (F4/80 antibody) at dilution of 1:12000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 29414-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 29414-1-AP (F4/80 antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 29414-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 29414-1-AP (F4/80 antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat spleen using 29414-1-AP

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 29414-1-AP (F4/80 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 29414-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 29414-1-AP (F4/80 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 29414-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 29414-1-AP (F4/80 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 29414-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29414-1-AP (F4/80 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 29414-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29414-1-AP (F4/80 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 29414-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 29414-1-AP (F4/80 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 29414-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 29414-1-AP (F4/80 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 29414-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 29414-1-AP (F4/80 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 29414-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 29414-1-AP (F4/80 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat spleen using 29414-1-AP

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 29414-1-AP (F4/80 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse small intestine using 29414-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 29414-1-AP (F4/80 antibody) at dilution of 1:12000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse small intestine using 29414-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 29414-1-AP (F4/80 antibody) at dilution of 1:12000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 29414-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 29414-1-AP (F4/80 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 29414-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 29414-1-AP (F4/80 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse spleen using 29414-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using F4/80 antibody (29414-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse spleen using 29414-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using F4/80 antibody (29414-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse liver using 29414-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using F4/80 antibody (29414-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse liver using 29414-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using F4/80 antibody (29414-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: mouse spleen tissue, mouse liver tissue, rat spleen tissue, rat liver tissue, mouse lung tissue, mouse heart tissue, mouse brain tissue, mouse small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse spleen tissue, mouse liver tissue

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:8000-1:20000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 30 publications below
• IF: See 29 publications below

Product Information

29414-1-AP targets F4/80 in WB, IHC, IF-P, ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: F4/80 fusion protein Ag30149
• Full Name: EGF-like module containing, mucin-like, hormone receptor-like sequence 1
• Calculated Molecular Weight: 102 kDa
• GenBank Accession Number: NM_010130
• Gene Symbol: F4/80
• Gene ID (NCBI): 13733
• RRID: AB_2918300
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Mouse F4/80, also named as EMR1 and Gpf480, is a 160kd cell surface glycoprotein which is a member of the EGF TM7 family. The F4/80 molecule is solely expressed on the surface of macrophages and serves as a marker for mature macrophage tissues, including Kupffer cells in liver, splenic red pulp macrophages, brain microglia, gut lamina propria and Langerhans cells in the skin. The function of F4/80 is unclear, but it is speculated to be involved in macrophage adhesion events, cell migration or as a G protein-coupled signaling component of macrophages.

Publications

Species	Application	Title
human	IHC,IF	Cancer Commun (Lond); Glutamine metabolic microenvironment drives M2 macrophage polarization to mediate trastuzumab resistance in HER2-positive gastric cancer; Authors - Xingbin Hu
mouse	IHC,IF	J Exp Clin Cancer Res; FAM171B stabilizes vimentin and enhances CCL2-mediated TAM infiltration to promote bladder cancer progression; Authors - Wei-Min Hu
rat	IHC,IF	Mater Today Bio; Mussel-inspired collagen-hyaluronic acid composite scaffold with excellent antioxidant properties and sustained release of a growth factor for enhancing diabetic wound healing.; Authors - Yong Wang
mouse	IF	ACS Appl Mater Interfaces; Catalysis-Mediated Male Contraception through Black Phosphorus Nanosheets; Authors - Lirong Yi
rat	IF	Mater Today Bio; Intracellular hydrogelation of macrophage conjugated probiotics for hitchhiking delivery and combined treatment of colitis; Authors - Jingzhe Wang
mouse	WB,IHC	Ecotoxicol Environ Saf; Triclosan induces liver injury in long-life exposed mice via activation of TLR4/NF-κB/NLRP3 pathway; Authors - Yuxuan Peng