F4/80 Polyclonal antibody

F4/80 Polyclonal Antibody for IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG


Mouse, Rat and More (4)





Cat no : 28463-1-AP


Adgre1, DD7A5 7, EGF TM7, Emr1, F4 80, F4/80, Gpf480, Ly71, TM7LN3

Tested Applications

Positive IHC detected inmouse spleen tissue, rat spleen tissue, rat liver tissue, mouse lung tissue, mouse heart tissue, mouse brain tissue, mouse liver tissue, mouse small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse spleen tissue, mouse liver tissue, RAW 264.7 cells
Positive FC detected inRAW 264.7 cells

Recommended dilution

Immunohistochemistry (IHC)IHC : 1:4000-1:16000
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28463-1-AP targets F4/80 in WB, IF, FC, IHC, ELISA applications and shows reactivity with Mouse, Rat samples.

Tested Reactivity Mouse, Rat
Cited Reactivityhuman, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen F4/80 fusion protein Ag28252
Full Name EGF-like module containing, mucin-like, hormone receptor-like sequence 1
Calculated Molecular Weight 102 kDa
GenBank Accession NumberNM_010130
Gene Symbol Emr1
Gene ID (NCBI) 13733
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Mouse F4/80, also named as EMR1 and Gpf480, is a 160-kDa cell surface glycoprotein which is a member of the EGF TM7 family. The F4/80 molecule is solely expressed on the surface of macrophages and serves as a marker for mature macrophage tissues, including Kupffer cells in liver, splenic red pulp macrophages, brain microglia, gut lamina propria and Langerhans cells in the skin. The function of F4/80 is unclear, but it is speculated to be involved in macrophage adhesion events, cell migration or as a G protein-coupled signaling component of macrophages.


Product Specific Protocols
IHC protocol for F4/80 antibody 28463-1-APDownload protocol
IF protocol for F4/80 antibody 28463-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Kannan (Verified Customer) (06-13-2023)

AR using EDTApH9. overnight incubation of primary antibody at 4c.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: mouse uterus