F2RL3 Polyclonal antibody

F2RL3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 25306-1-AP

Synonyms

Proteinase-activated receptor 4, Proteinase activated receptor 4, PAR-4, PAR4, PAR 4

---

Validation Data Gallery

• 

  WB analysis using 25306-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25306-1-AP (F2RL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 25306-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 25306-1-AP (F2RL3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 25306-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 25306-1-AP (F2RL3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse lung using 25306-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 25306-1-AP (F2RL3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 25306-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 25306-1-AP (F2RL3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: mouse lung tissue, mouse pancreas tissue
• Positive IHC detected in: mouse lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

25306-1-AP targets F2RL3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: F2RL3 fusion protein Ag20801
• Full Name: coagulation factor II (thrombin) receptor-like 3
• Calculated Molecular Weight: 385 aa, 41 kDa
• Observed Molecular Weight: 41 kDa
• GenBank Accession Number: BC074782
• Gene Symbol: F2RL3
• Gene ID (NCBI): 9002
• RRID: AB_2880022
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Coagulation factor II receptor-like 3 (F2RL3) encodes a member of the protease-activated receptor subfamily, also known as protease-activated receptor 4 (PAR4), which takes partin platelet activation, intimal hyperplasia and inflammation (PMID:34284820). An absence of PAR4 in mouse models results in impaired hemostasis and a protection against pulmonary embolism, and a small number of missense coding variants in F2RL3 that alter platelet aggregation and function have been described(PMID:35012325). The calculated molecular weight and observed molecular weight of F2RL3 are both 41 kDa.

Publications

Species	Application	Title
human	WB	Oxid Med Cell Longev; Combination of Colchicine and Ticagrelor Inhibits Carrageenan-Induced Thrombi in Mice.; Authors - BuChun Zhang
mouse	WB	Front Oncol; Tumor Suppressor Par-4 Regulates Complement Factor C3 and Obesity.; Authors - Nathalia Araujo
mouse	WB	Neurosci Bull; Antagonism of Protease-Activated Receptor 4 Protects Against Traumatic Brain Injury by Suppressing Neuroinflammation via Inhibition of Tab2/NF-κB Signaling.; Authors - Jianing Luo

Related Products

PAR2 Polyclonal antibody

12160-1-AP

F2 Polyclonal antibody

24295-1-AP